Inoculation for Anthrax and Quarter 111. 
463 
Of those inoculated intravenously on November 12th, i.e. 
Nos. 1, 2, 3 and 4, neither has appeared to suffer in the slightest 
degree. Of the four unprotected, one A. died of the disease 
taken naturally while grazing with the others ; two, C. and D., 
died of the disease produced by the test inoculation on the 
25th ; and one, B., through contracting the disease from the test 
inoculation, survived in a manner we have frequently had 
occasion to notice. It was suggested to Major Percy that after 
perfect recovery B. should again be inoculated with virulent 
matter, with a view of ascertaining how far his mild attack had 
afforded him immunity, as it is usual for mild or resisted attacks 
of such diseases to act in this fashion ; but it was decided that it 
could not be conveniently carried out. 
However, to test this view, we utilized the mild case pre- 
viously referred to as occurring near Nottingham. On Decem- 
ber 29th, after perfect recovery of the bullock, sixty minims 
of muscle-juice taken fresh from a fatal case of Quarter 111 
were injected into its thigh. Following this there was little 
general and no appreciable local disturbance. The temperature 
four hours after the operation rose to 104'2, in eighteen hours 
it became normal, and from this time the animal fattened 
rapidly. 
Simultaneously with the bullock, two guinea-pigs were in- 
oculated each with two drops of muscle-juice from the same 
source, and died within 25 hours, post-mortem examination 
revealing conditions characteristic of Quarter 111. 
In order to prove the practicability of the intravenous method 
of protective inoculation on December 22nd, Professor Pen- 
berthy, with aid of Mr. Tomes, the bailiff, inoculated 24 young 
cattle at Hodnet Hall. On this occasion, the injection was 
done in every case by piercing direct through the skin into the 
vein. The injected virus was taken from a heifer which had 
died some hours previously. The operation lasted about 1 hour 
20 minutes. There was. Major Percy states, no sign of indis- 
position in the animals after the inoculation, and up to the date 
of writing this report there has been no case of Quarter 111 
amongst these calves. 
It may be interesting to add here the statement that each 
of the seven animals which died during the carrying out of 
the experiments at Hodnet Hall had been duly setoned and 
drenched. 
The practical outcome of this work appears to us to be that 
it has made clear the fact of the communicability by inoculation 
of the disease known in England as Quarter 111, and its iden- 
tity with that spoken of by both Arloing, Cornevin and Thomas 
as " Charbon Symptomatique." Without further proof of its 
