206 Report on Plcuro-Pneumonia and Foot-and-Moutli Disease. 
2. On the 22nd of June, the same animals were again 
operated on by submucous puncture, the material employed 
being diseased epithelium taken fresh from unruptured vesicles. 
3. On the 29th, the same two cows were fed with hay which 
had been steeped in the saliva of diseased animals. It was given 
within about three hours after its collection. 
4. On July 11, the same method of inoculation as in Experi- 
ment 1 was again employed for the infection of two calves. 
5. On Aug. 3, the same calves had their gums again scarified 
and saliva applied, and were then fed with a small quantity of 
hay soaked in the saliva of diseased animals. 
6. On the 15th of August they were again fed with hay wet 
with the saliva of diseased animals. 
7. In the following instances infection was attempted by the 
method of subcutaneous injection of saliva, in addition to the 
other modes of operating in the animals referred to : 
In Experiment 1 . . One animal. 
55 2 . . ,, „ 
4 
55 1 . . ,, ,, 
55 -».*<.* 55 55 
8. On Oct. 28, the young calf, two months old, was injected 
subcutaneously with saliva. 
9. The exudation material derived from the matrix of a 
diseased hoof, was mixed with neutral and non-irritating saline 
solution, and injected subcutaneously in three animals, each 
receiving three separate punctures, viz., one in the ear, the 
second in the perineum, and the third at the border of the hoof 
in the heel of the fore foot. 
The general result of these experiments has been, that in 
every instance, without exception, the effect of subcutaneous 
puncture has been to produce a limited abscess, containing a 
slough, with scarcely any extension of inflammation to neigh- 
bouring parts. In the case in which the punctures were made 
at the border of the hoof, no effect could be observed. 
With reference to the general question of the communica- 
bility of the disease, the results must as yet be regarded as 
negative. 
It is proposed to continue the investigation as follows : — 
As regards Foot-and-Mouth Disease, the experiments will be 
continued on the same plan, but new methods of infection on 
other animals will be tried. 
As regards Pleuro-pneumonia, we propose 
1. To repeat on those animals that have not yet been used 
for experiments on Pleuro-Pneumonia the injection into the 
circulation. 
2. To repeat on some the subcutaneous injection. 
