94 
The Tubercle Bacithis. 
it reached 107f° Falir. in two hours. After nine injections 
death resulted, and at the post-mortem examination the spleen, 
liver, and lungs were found to be diseased, the spleen and liver 
being almost solid masses of tubercular deposit. In the other 
guinea-pig, inoculated with human tuberculosis, the disease is 
progressing, but the animal is, at the time of writing, still living. 
With the exception of a very slight rise in temperature, the 
healthy guinea-pig was not affected by the injection. In France 
Jaccoud inoculated a guinea-pig with Koch's liquid, and subse- 
quently inoculated tubei'cular virus ; and the result led him to 
conclude that previous impregnation of the system with Koch's 
liquid had in this case no preventive action. Dujardin-Beau- 
metz made similar experiments with similar results. Animals 
were inoculated with Koch's tuberculine and then with the virus 
of tubercle ; in other cases he inoculated with tubercle first and 
afterwards with tuberculine. In both series the animals died 
of tuberculosis. 
Effect of Tuberculide on Cattle. 
In the Veterinary Institute at Dorpat, Gutmann injected 
three tubercular cows with tuberculine. In all three animals the 
temperature began to rise about eleven hours afterwards, and in 
all during the "reaction " there was some difficulty of breathing 
and loss of appetite. The disease was diagnosed partly by the 
physical examination, and partly by the detection of bacilli in 
the bronchial mucus and in the milk, and by changes in the lym- 
phatic glands. Gutmann concluded that Koch's liquid was an 
aid to diagnosis. At the Eoyal Veterinaiy College the writer 
injected a cow, supposed to be tubercular, on two occasions with 
doses of two and three centigrauimes. The temperature, takSn 
hourly, remained normal, but increased cough was noted. A 
third dose of fifty centigrammes was followed by a negative 
result, and a fourth dose of one gramme of the liquid produced 
a slight rise in temperature. The case is still under observation. 
In a subsequent article the writer hopes to say more on 
the question of cure, and the effect on animals in relation to 
diagnosis. 
Edgar M. Crookshank. 
