Experimental Investigation on Anthrax, Sfc. 31 
ficially in organic fluids ; and I have been able to prove that it 
is capable of gradual attenuation, even to the degree of complete 
destruction of its virulence, and that it may thus be gradually 
modified, so that a certain degree of poisonous activity may be 
attained at will. As yet I have not been able to make any 
complete series of experiments on bovine animals by this method, 
as it seemed to me desirable first to obtain very precise results 
with smaller and less costly animals. 
Inoculation Experiments. 
In order to give a complete survey of the experiments on 
bovine animals, it will be necessary to refer to two of the cases 
given in my previous Report, as some of the experiments here 
detailed were performed upon the same animals. Case I. of the 
former Report will be mentioned as A, and Case III. as B, 
The remainder are fresh cases, and will be described as C, D, 
&c. I shall first mention the experiments upon C and D, two 
young heifers of about the same age and size, both in good con- 
dition, upon which comparative observations were made. 
Heifer C : Experiment 1. — The first inoculation was made 
with the cultivated Bacillus anthracis of the third generation, 
prepared in the manner described in my previous Report. One 
minim of this fluid, containing rods and spores of Bacillus 
anthracis, was injected at 3 P M. beneath the skin of the shoulder. 
No symptoms were observed until the forenoon of the following 
day, when the animal was found to be stupid and drowsy, with 
rapid respiration. Some local swelling was observed at the seat 
of inoculation, and this extended on the following day to the 
whole shoulder and forelimb. The course of the symptoms will 
best be seen by the following table of temperatures : — 
Day of 
Inoculation. 
8 to 9 A.M. 
1 to 2 P.M. 
7 to 8 P.M. 
0 
0 
1 
10°l-6 
2 
i6i-4 
105 -2 
105-4 
3 
105-8 
105-8 
106 '6 
4 
104* 2 
104-8 
106 
5 
102-8 
103-4 
6 
101 
102-4 
7 
101-2 
161-2 
101-6 
8 
101 
The symptoms, both local and general, closely corresponded 
with the course of temperature here described. During the 
