32 Report on an Experimental Investigation on 
persistence of high temperature (over 104° F.) there was great 
dulness and prostration, and loss of appetite, and a considerable 
local swelling. With the subsidence of the fever there was 
rapid recovery of general condition and appetite, and a dimi- 
nution of the local swelling. In the course of two or three days 
the animal appeared to have entirely recovered. 
It must be observed that neither in this nor in any other case 
was there any pustule upon the surface of the skin ; there was 
local swelling, redness, and tenderness, and general inflammatory 
tfidema, but nothing like the malignant pustule of the human 
subject. Nor had the local swelling any resemblance to the 
emphysematous swelling of black quarter. Blood from the ear 
could not be found to contain any bacilli ; nor did its normal 
characters appear affected. 
An interval of ten weeks was now allowed to elapse, during 
which one inoculation was made with the thirteenth generation 
of cultivated virus — material which, as I shall show later, is abso- 
lutely innocuous, and in this case produced no effect whatever. 
At the end of the ten weeks, the animal being apparently in per- 
fect health, it was again inoculated ; this time from a guinea-pig 
which had died of anthrax. The spleen of the guinea-pig, 
which was much swollen and very soft, and swarmed with 
bacilli, was reduced to a pulp and mixed with a drop or two of 
water, and the entire quantity injected beneath the skin of the 
flank. Not the slightest symptom, either local or general, fol- 
lowed this inoculation : there was, of course, a small swelling 
produced by the presence of the injected fluid, but neither 
redness nor tenderness, nor in fact more effect than would be 
produced by the injection of so much water. 
We may now turn to the experiments in the next case, leaving 
the further observations on heifer C for comparison with those 
on heifer D at a later period. 
Heifer D, an animal of the same age and size as heifer C, 
was kept under exactly similar conditions. Five days after the 
inoculation of heifer C with the 3rd generation of cultivated 
bacillus, heifer D was inoculated with the 8th generation in 
active growth. A very slight rise of temperature (1° F.) was 
observed on the following day, but the same had occurred before 
without any obvious cause, and there were no symptoms. Sub- 
sequent experiment rendered it almost certain that the material 
was inert. A further inoculation a week later with the 7th 
generation was likewise without definite effect, beyond a small 
local swelling. When heifer C was inoculated with the 13th 
generation, heifer D was also similarly inoculated, but this 
