Anthrax, Sfc, made at the Brown Institution. 
35 
performance of the experiment ; but I had attached too great an 
importance to the fact of previous inoculations. 
The onset of the symptoms took place in about twelve hours 
from the inoculation, but it was not until twenty hours that the 
case appeared at all critical. From that time, however, the 
animal appeared completely prostrate, and scarcely able to move, 
and was apparently at the point of death. There was much 
local swelling, which involved the shoulder and forelimb. On 
the fifth day, when the general condition had considerably im- 
proved, this swelling was so considerable that it was found 
necessary to make incisions into it, and to treat it locally with 
hot fomentations, under which rapid improvement and recovery 
ensued. It may be to these incisions that the slight after-rise 
of temperature was due. 
The nature of this local swelling is a matter of some interest. 
As in all the cases which I have seen, it consisted of a gelatinous 
looking oedema of the subcutaneous tissue and fasciae. I was 
unable to discover bacilli in the fluid expressed from it. 
Perfect recovery rapidly ensued. The experiment, severe 
as it seemed, can hardly be regretted, as it afforded so com- 
plete a test of the protection afforded by the inoculation in the 
heifer C. 
The evidence of the protective value of inoculation as a 
safeguard against subsequent inoculation, at any rate within a 
limited period, having been so conclusively established by these 
and previous experiments, I had no hesitation in submitting the 
same two animals to another comparative test a month later. 
The opportunity was afforded by the occurrence of an outbreak 
of splenic fever at Harden, of which I shall give particulars, 
and which arose in connection with the washing of infected 
wool and the distribution of the waste water as sewage upon the 
farm. Blood from the spleen and the pericardial serum of a 
cow which died of the disease, were employed. The nature 
and virulence of the disease were fully determined by inoculation 
of rodents with the same fluids, but it may be remarked that the 
blood from the spleen was more active than the pericardial serum. 
Heifer C was inoculated with about 20 minims of blood from 
the spleen ; heifer D, with a similar quantity of pericardial serum, 
the injections being made in the same manner as before. 
Although in the case of the heifer C, a rise of temperature 
of i° to 1° on the following evening was registered, there were 
absolutely no symptoms in either case ; and it may be that this 
slight rise was rather due to the greater solidity of the material 
employed and consequent local irritation, than to any general 
absorption of the poison. 
D 2 
