36 Report on an Experimental Investigation on 
Inoculation of Heifers C and D witli Fluids from a Cow whicli 
DIED of Anthrax. 
Day of 
Inoculation. 
Heifer C. 
Heifer D. 
8 to 9 A.M. 
8 to 9 P.M. 
8 to 9 A.M. 
8 to 9 P.M. 
1 
101-8 
102 
2 
162-2 
103-4 
161-6 
102 
3 
102-4 
103 
101-4 
101-8 
4 
102-4 
102-4 
101-6 
101-8 
5 
101-6 
102-2 
102 
101-8 
6 
101-8 
102 
101-4 
101-4 
7 
101-8 
102-8 
101-8 
101-6 
8 
101-8 
101-4 
9 
101-G 
101-4 
We must now return to the animals previously inoculated, 
A and B. In the case of A, as I mentioned in my previous 
Report, very severe tests had been applied to test the efficacy of 
the first inoculation as a protection against subsequent inocula- 
tion, and the result showed that, within the limited period over 
which the experiments extended, the protection conferred was 
complete. It was therefore decided to wait for a considerable time 
before submitting this animal to any fresh tests, lest it should be 
objected that the protection conferred was only temporary and 
was maintained by the subsequent inoculations, although they 
produced no visible effect. Similarly, with B, some little time 
was allowed to elapse before the application of any severe test, 
one or two inoculations with the cultivated virus alone being 
used. These, which were with the seventh and eighth genera- 
tion of the bacillus, produced no effect whatever. Five months 
having now elapsed, one drachm of blood from the heart of 
the sheep which had died of anthrax, swarming with bacilli, 
was injected beneath the skin of the shoulder without any effect. 
It must be mentioned that the temperature of this animal was 
found to be subject to such variations that the records do not 
give a correct idea of the results of the experiment. 
The subsequent observations on these animals consisted 
merely in placing them under known conditions of contagion. 
These will be described when I have mentioned the circum- 
stances under which this was possible, afforded by the occur- 
rence of a considerable number of cases of wool-sorters' disease 
at Bradford. It will be necessary to give a brief account of 
this outbreak. 
It had been known for many years that the operatives in 
large wool factories wheie foreign wools were much used were 
liable to a form of fatal blood-poisoning, which appeared to be 
