270 
Experiments on Anthrax conducted at 
available for these experiments, and therefore the inoculation of 
cattle with the original anthrax blood could not be tried. 
On March 25th the spleen of a guinea-pig that died from 
inoculation with anthrax blood was triturated in a mortar with 
a small quantity of ^ per cent, saline solution, and the resulting 
liquid, containing large numbers of bacilli, was filtered and used 
to inoculate a calf about six months old. About three drops of 
the fluid was introduced into the cellular tissue in front of the 
shoulder, near the course of the jugular vein. On the following 
day a small nodule was felt at the seat of puncture, but no 
general symptoms were visible. On the 27th, the second day 
after inoculation, the temperature had risen from 102° Fahr. to 
103°* 6. The animal refused its food, and showed well-marked 
twitching or shivering of the superficial muscles. The tempera- 
ture still further rose and reached its maximum, 105°'2. On 
the third day the calf was in a very weak and prostrate con- 
dition ; when moved it appeared stiff and unable to walk, 
staggered on its hind legs, and seemed about to fall. These 
symptoms were accompanied in the later part of the day by 
diarrhoea, and the animal seemed dying. 
On the fourth day the temperature had fallen considerably, 
the animal was more lively and fed a little ; the diarrhoea still 
continued. 
March SOth. — The fifth day after the inoculation the animal 
was quite well ; temperature normal. 
As a check-experiment in this case some of the material with 
which the calf was inoculated was used to inoculate a guinea- 
pig, which died in about forty-eight hours. 
3fay 1th. — A yearling heifer was inoculated with blood from 
a guinea-pig that died as the result of inoculation with dried 
anthrax blood obtained February 18th. In this case the blood 
was diluted with its own volume of saline solution, and five 
drops of this mixture were injected directly into the posterior 
auricular vein. 
On the following day the animal was ill and refused its food. 
The temperature had risen from 101°"8 Fahr. to 103°'2 ; there 
was twitching of the muscles and constipation. 
The temperature in this case reached its maximum, 106°* Fahr,, 
on the evening of the second day, and from the prostrate con- 
dition of the animal it was expected to die during the night, 
instead of which the temperature declined to 104'2°. The con- 
stipation was followed by diarrhoea, and the animal began to take 
food. On the fourth day the temperature was nearly normal, 
and the animal fairly well. 
At the same time another yearling heifer was inoculated by 
the introduction of some of the same solution into the cellular 
