the Brown Institution. 
271 
tissue ; and In this case the symptoms produced were of tlie 
same nature, but were less severe, and developed more slowly. 
The maximum temperature, 105^-2, was not reached till the 
evening of the third day. 
May l^th. — The six-months-old calf inoculated March 25th, 
and the two yearlings inoculated May 7th, were again used for 
inoculation with blood from a guinea-pig that died as the result 
of inoculation with anthrax cultivated to the third generation 
in grains' infusion. 
In the case of the calf, three drops of blood mixed with some 
saline solution were injected into the posterior auricular vein, 
and in the two others it was introduced into the cellular tissue. 
In the calf the symptoms were very severe ; the maximum 
temperature, 106°*2, was reached on the morning of the third 
day, and no material decline was noted until the evening of the 
fourth day. During this time the animal refused food, but 
showed considerable thirst ; was in an extremely prostrate con- 
dition, and suffered from diarrhoea. In the other two animals 
the symptoms were less severe, developed more slowly, and 
declined earlier. 
June 10th. — The same three animals were again inoculated 
with anthrax cultivated to the third generation in grains' 
infusion. 
In two, the same symptoms were produced as when they were 
inoculated with the anthrax blood ; but in the third animal, 
from some unexplained cause, no general symptoms followed 
the inoculation, but a small abscess formed at the seat of 
puncture. 
III. Anthkax Cultivation Experiments. 
In the very serious outbreak of anthrax on Mr. Mason's farm 
at Rigsby, near Alford, Lincolnshire, the affected cattle had been 
fed on grains brought from a neighbouring brewery ; and as this 
was the only difference between the feeding of the affected and 
unaffected animals on the farm, it was at first supposed that the 
grains had produced the disease. Four cattle were fed for several 
days at the Brown Institution with some of the grains sent up 
by Mr. Mason for that purpose, but none of them showed any 
signs of being in the least affected. 
The fluid expressed from the grains was used to inoculate 
some guinea-pigs in the subcutaneous cellular tissue, but the 
only result was local swelling and the formation of small 
abscesses at the seat of puncture. Although these experiments 
gave negative results, it was considered advisable to try whether 
the anthrax bacillus could live in such a medium as grains, 
