Prevention of Splenic Fever, SjC, at the Broion Institution. 281 
Fig. 4. — Bacillus anthracis tmgnified 1500 diameters. 
1. A short rod (8 ix long) in which the protoplasmic contents have divided previous to fission of the 
rod. 2. A clear rod containing only spores. 3. A jointed filament composed of three segments, in 
one of which fission is commencing, in another a spore has formed, whilst another is empty. 
4. Part of tilament composed uf short homogeneous rods united by a narrower sheath. 5, 6, 7. Parts 
of long filaments showing various arrangements of the protoplasm and spores. 8. From another 
^ filament in which spore formation is proceeding more regularly. Commencing fission is seen at 
some parts of the filament. 9. Part of filament completely filled with regularly arranged spores. 
10. From another cultivation showing sporule germinatmg into a short rod. 
(It must be stated that the space between the sheath and the 
protoplasmic contents did not exist in the original drawing, 
and that in the other rods it has been necessarily somewhat 
exaggerated in the woodcut.) 
I have thought it well thus briefly to describe the changes 
usually observed, in order to render clear the frequent references 
to these various conditions in describing the experiments. 
Details of Experiments on Inoculation of Bovine Animals with 
Anthrax poison (Bacillus anthracis) transmitted through 
Rodents. 
Case I. — A steer, in good health and condition, was kept 
under observation for three weeks, in order to ascertain its 
normal temperature. It was then inoculated with a small quan- 
tity of fluid which had been obtained by cultivation of the 
Bacillus anthracis in aqueous humour, and which swarmed with 
rods and spores of bacillus. The fluid thus used was the second 
generation of the cultivation of spleen of a guinea-pig which 
died of anthrax. This guinea-pig was the third of a series, 
through which the disease was transmitted from the cow by 
inoculation. I have already described the method followed in 
these experiments. 
For about twenty-four hours no symptoms were observed, 
there was only very slight swelling at the seat of inoculation. 
