282 Report on an Inquiry into the Nature, Causes, and 
On the morning of the second clay, i.e., about forty hours after 
the inoculation, the temperature (in the rectum) was found to 
be 104° Fahr., the normal temperature having been 100° to 
101° Fahr. The animal was drowsy and stupid and fed badly, 
but no rigour was observed, nor any special symptoms. On the 
evening of the same day, the temperature was 102 '4° Fahr., but 
on the following day it rose to 106'4° Fahr., and continued to 
vary between 105° and 107° for three days, during which time 
the animal was seriously ill, and at times seemed hardly likely 
to recover. On the eighth day, however, the temperature fell to 
104°, and on the ninth to 101°, after which the animal speedily 
recovered and continued perfectly well. 
I was unable to discover any Bacilli in a drop of the blood 
examined, but I do not attach much importance to this fact as 
any proof that they were not present, for I have often failed to 
find them in the blood of the general circulation in smaller 
animals at a period before death, when they must have existed 
in the blood in some organs. 
The animal, having completely recovered, was again inoculated 
a second time some weeks from the first inoculation. During 
the interval after subsidence of the fever produced by the first 
inoculation, the temperature was at no time higher than 101'4°, 
ranging between 100° and 101°. 
This second inoculation was made directly with the fresh 
spleen of a guinea-pig which had died of anthrax, simply 
rubbed down with saline solution to make it more fluid. One 
gramme of this fluid was injected with a hypodermic syringe 
into the right side about the elbow. The fluid swarmed with 
rods. 
In the evening the temperature was 102°. Next day the tem- 
perature was 102*4° morning, and 102'6° evening, and the animal 
showed some indisposition and took food badly. On the third 
day there was complete recovery of appetite, and the temperature 
became normal. The subsequent temperatures will be seen by 
reference to the appended table. (See page 284.) 
The third inoculation was made a week after the second. 
The animal had completely recovered in every respect, and 
it was desirable to test the power of resistance acquired by 
the previous inoculations, and to compare the result with a 
simultaneous inoculation of another animal with the same 
material. 
In this third inoculation the poison was obtained from a guinea- 
pig which had died of anthrax derived from the horse. The 
spleen having been removed shortly after death, was rubbed 
down with saline solution, and one-half of the fluid injected into 
