Prevention of Splenic Fever, S^'c., at the Broivn Institution. 283 
the steer, the other half into the cow (Expt. No. 2). No result 
Avhatever followed in the case of the steer, with the exception 
that on the evening of the following day the temperature was 
102°. But the appetite was unaffected ; the slight local swelling 
produced by the inoculation speedily subsided. 
Fourth inoculation. — A period of three weeks having elapsed 
after the third inoculation, during which the animal was in 
good health, a fourth inoculation was made, A guinea-pig, 
inoculated with blood from the guinea-pig used for a previous 
experiment, which had been dried and kept in a sealed tube 
for some weeks, died of anthrax. The spleen was consider- 
ably enlarged (an unusual occurrence in the guinea-pig), and 
very soft, and the fluid exuding from the cut surface swarmed 
with Bacilli. The whole spleen was rubbed down with about a 
drachm of water, and nearly the whole of the fluid with the 
particles of the spleen in suspension was injected by means of a 
hypodermic syringe beneath the skin of the shoulder. The 
result of this inoculation, so far as general symptoms are con- 
cerned, was absolutely nil, not the slightest rise of temperature 
or failure of appetite being produced, and the animal subse- 
quently remaining in perfect health. 
Fifth inoculation.- — -Fourteen days having elapsed since the 
previous inoculation, during which the temperature of the ani- 
mal had continued absolutely normal, on no occasion exceeding 
101° Fahr., another inoculation was made, the material on this 
occasion being derived from a sheep, which died of anthrax (see 
Case IV.). The bloody fluid which flowed from the cut surfaces 
of the lung was collected, and about one drachm injected beneath 
the skin of the right shoulder. The fluid injected contained a 
large number of very long bacillus rods, and caused the usual 
symptoms of anthrax in a guinea-pig inoculated at the same 
time. 
On the day following the inoculation, the part was consider- 
ably swollen, the animal was rather dull, and did not take food 
so well as usual, but there" were no other symptoms. There was 
some rise of temperature — 103"4° in the morning, 103*6° mid-day, 
and 104° evening. Next day there was still some slight loss of 
appetite, but the temperature was normal, and subsequently the 
animal continued in perfect health. A slight swelling remained 
at the seat of inoculation, which subsequently changed into a 
small abscess. This was opened, and discharged a small quantity 
of ichorous pus. 
This animal is still being kept, awaiting the opportunity of 
making the crucial experiment of direct inoculation from a case 
of anthrax in the cow. 
