286 Report on an Inquiry into the Nature, Causes, and 
Heart : numerous small ecchjmoses and petechiae beneath 
the endocardium, especially that of the left ventricle, and also in 
the musculi papillares. Some also more deeply in the muscular 
tissue, and one or two beneath the pericardium. 
Lungs appeared healthy and pale. 
On microscopical examination, the blood was found to con- 
tain very large numbers of rods of Bacillus anthracis, and the 
tissue both of the spleen and the other organs also swarmed with 
them. The sanious discharge from the nostrils also contained 
them in great abundance. 
The foetal calf, of about four months, appeared healthy. All 
its organs were carefully examined for Bacilli, but none were 
discovered. 
It will be observed that this experiment differed from the 
first case in being inoculated direct from the guinea-pig instead 
of with cultivated fluid, in the original source of the material, 
viz. the horse, and in the fact that the animal was old and 
emaciated and in-calf. The latter points appear to me to be of 
more importance than the former, as the animal in Expt. I. 
was inoculated in the same way and with the same material 
without any symptom being induced. 
Case III. — A calf 6 months old was inoculated with the fourth 
cultivation of anthrax Bacillus, derived from the guinea-pig. No 
symptoms whatever were produced. The temperature had been 
somewhat irregular previous to the inoculation, owing to an 
attack of diarrhoea. On the ninth day from the first inocula- 
tion, the animal appearing perfectly well, it was again inocu- 
lated with the first cultivation direct from the spleen, which had 
been kept sealed up for a fortnight, and swarmed with spores 
of Bacilhis anthracis. To test the virulence of the material, a 
mouse was inoculated at the same time with a very minute 
quantity, and was found dead the next day, about 20 hours from 
the time of inoculation. The spleen of the mouse was found to 
be crowded with rods of the usual character. 
On the day following inoculation the part was somewhat 
swollen, but there was no perceptible rise of temperature until 
the second day, when it rose to 106° morning, and 106'4° even- 
ing. For the ensuing four days the elevation of temperature 
persisted, with variations from 105"4° to 106"4°, there was con- 
tinued swelling of the shoulder, which extended, and, together 
with this, loss of appetite. On the seventh day, the morning 
temperature was 103'8°, but as the swelling persisted and 
showed no sign of subsiding, an incision was made into it, 
and fomentations applied. The subcutaneous connective tissue 
was found to be infiltrated with colourless, somewhat gela- 
tinous, serous exudation. On microscopic examination no 
