Prevention of Splenic Fever, Sj-c., at the Brown Institution. 287 
Bacilli or Bacteria were found either in this serous fluid or in 
the blood. 
The animal speedily improved, the swelling subsided, and com- 
plete recovery occurred. A sufficient time has not yet elapsed 
lor further inoculations to be made. 
Case III. — Calf Srx Months Old. 
Experiment I. 
Day of 
Temperature in Kectum. 
Observa- 
Remarks. 
Morning. 
H'oon. 
Evening, 
o 
o 
0 
103- 
■ 
/First inoculation, -with 4th genera- 
1 
\ tion of cultivated material. 
2 
104' 
103-6 
3 
104- 
164-6 
105- 
4 
103-6 
104-2 
5 
104- 
103-4 
6 
103- 
103-8 
7 
103- 
103-8 
8 
102- 
103- 
9 
103- 
103- 
(■Second inoculation, with first geue- 
( ration. 
10 
103- 
103-2 
11 
106- 
105-6 
106-4 
12 
105-4 
106-4 
105-8 
13 
105-4 
105-6 
105-8 
14 
•106-4 
106-2 
105-8 
15 
106-2 
106-4 
16 
103-8 
103 8 
104- 
17 
103-4 
103- 
102- 
f Local s-welling subsiding. Eats 
1 -well. 
18 
102-4 
102- 
102- 
19 
101- 
101-2 
101-4 
Quite well. 
Inoculation of Sheep, with cultivated Bacillus antkracis, in the 
form of Spores. 
Some doubts having naturally arisen as to whether the culti- 
vated material used in the preceding experiments could be re- 
garded as representing the anthrax poison in a virulent form, 
and an opportunity arising for inoculation of a sheep, I thought 
it desirable to test the material upon this animal. And as the 
disease is liable to be communicated to sheep, and in some 
countries frequently forms a fatal epidemic amongst them, the 
question whether the poison was modified in its action upon 
them by transmission through rodents appeared important. 
The following experiment was therefore performed : — 
Case IV.—A young sheep was inoculated with a small quan- 
tity of the cultivation of the fourth generation, which had been 
