300 Beport cn an Inqtiiri/ into the Nature, Causes, and 
muscles of the thigh and abdomen were also swollen, of very 
black colour, and contained numerous ecchymoses. The spleen 
appeared perfectly healthy, and not apparently enlarged. 
On examining the serum from the swollen connective tissue 
of the thigh, it was found to contain ordinary bacteria and micro- 
cocci, the bacteria in very active movement. Serous fluid, which 
was somewhat blood-stained, obtained from the centre of affected 
muscles, also contained a few moving bacteria of the common 
form. The blood from the heart appeared to be perfectly 
healthy, and contained neither bacteria nor micrococci of the 
ordinary form. That from the spleen swarmed with bacteria 
and micrococci, a few of the rods being quiescent, but even 
these having the form of ordinary decomposition bacteria. The 
lungs appeared perfectly natural. I could not collect any fluid 
in the serous cavities. 
In this case, then, there were none of the usual characters of 
anthrax, and neither bacillus rods nor spores were to be dis- 
covered in the spleen or tissues ; but there are two note- 
worthy points, viz., that the emphysematous swelling of the 
inoculated limb and of the abdominal wall, the almost gangre- 
nous state of the tissues and the black condition of the muscles 
were present, reproducing in this respect the clinical features ot 
the original disease. At the same time there was evidence that 
decomposition had already commenced during life, in the fact 
that the splenic tissue swarmed with bacteria. I have never 
found this before in an animal killed and examined at once. 
In the other inoculations I was unable to examine the tissues 
immediately after death. 
Experiment II. — A guinea-pig, inoculated in the leg with 
serum from the affected quarter at the same time as the pre- 
ceding, was found dead at 8'30 A.M. on October 11. I was 
unable to examine the body till 5 P.M. The whole of the 
quarter where the inoculation was made was greatly swollen, the 
hair and epidermis were detached with great ease, even by a 
slight touch. In the subcutaneous tissue of this limb and over 
a great part of the abdominal wall was a quantity of deeply 
blood-stained exudation. The muscles of the thigh were 
swollen and black, containing small spots of ecchymosis. The 
other hind limb was not affected. All the viscera and the 
serous cavities Avere apparently perfectly healthy, and the spleen 
was free from enlargement. 
The blood-stained serum from the subcutaneous tissue con- 
tained many red blood-corpuscles, a few ordinary moving rod- 
shaped bacteria and some micrococci, no still rods or spores. 
The peritoneal scrum contained no bacteria at all, nor did the 
blood from the heart. In the spleen were found only ordinary 
