Prevention of Splenic Fever, Sfc, at the Brown Institution. 301 
moving bacteria. In the pleural serum were a few longer 
moving rods, some measuring from 8 to 12 yu, in length, 
showing signs of incipient division, but not containing sjiores. 
Experiment III. — Another guinea-pig was inoculated at the 
same time with blood from the heart, which, it will be re- 
membered, contained spores resembling anthrax spores, and 
was also found dead thirty-three hours after the inoculation, 
having had a temperature ten hours after of 106°, and in the 
evening, twenty-two hours after inoculation, of 100'4''. The 
appearances, both naked-eye and microscopical, were precisely 
similar to those in the preceding : no Bacilli nor definite spores 
were found. 
Exjieriment IV. — A rabbit, inoculated in the leg with fluid 
from the spleen, presented on the following day a very marked 
swelling of the whole of the inoculated limb, much resembling 
that in the guinea-pigs. The temperature was 104° for the next 
two days, morning and evening ; on the third day it was 103° 
to 103*4°, on the fourth, 102-8°. The swelling gradually 
declined and the animal recovered. These experiments were 
not continued further, for at the time they appeared to me to 
indicate that the material employed must have been too much 
decomposed to be of value. But on reconsideration I am 
inclined to think that this may have been an error. 
In all four of these experiments the sequel was different, both 
from that of the inoculation of ordinary septic material, and 
from that containing anthrax Bacilli. In all there were the 
peculiar emphysematous swelling and the black condition of 
the muscles, with ecchymoses scattered through them, which 
are characteristic of and give the special feature to the disease 
known as black-quarter. In no other experiments with animal 
fluids have I seen anything at all similar produced. Moreover, 
it was produced alike with the blood from the heart, juice 
expressed from the spleen, and the serous exudate from the 
affected quarter. 
I would especially notice the fact that the spores found in the 
spleen gave no signs of growth ; that they were, in fact, not re- 
produced in the slightest degree in the animals inoculated. Now 
what has commonly been observed is that if material containing 
a quantity of anthrax spores is inoculated, anthrax Bacilli, being 
developed more rapidly in the body than other Bacilli, produce 
the symptoms of splenic apoplexy. Here we have evidently to 
deal with a poison equally rapid and fatal in its action in 
some cases, producing decomposition actually during life, but 
apparently hindering the development of Bacillus anthracis, if 
that organism is really present. 
Some other experiments, previously made, show that the 
