The Occurrence of Aspergillosis in the Ostrich in South Africa. 201 
{d) Ingestion of a bouillon-lactose-glycerine-culture to the ostrich and 
pigeon. 
(e) Ingestion of a portion of intestines and contents of intestines, 
collected from a chick which died from ingestion of a culture, to the 
ostrich. 
(/) Inoculation of the buccal mucous membrane, after scarification, 
with a culture to a chick aged a few days. 
(g) Contact with a chick which had been dosed with Aspergillus 
culture. 
In the following instances results were negative : — 
(a) Two sheep and 2 cattle injected intravenously on the 25/7/12 
remain apparently healthy to date, 10/9/12. 
{b) One fowl put in inhalation experiment 26/7/12 likewise remains 
apparently healthy to date 10/9/12. 
(c) Introduction of a portion of a culture (potato) into the trachea o 
two fowls has had no apparent ill-effects to date 10/9/12. Date of 
experiment 16/8/12 and 23/8/12 respectively. 
(d) Three 9-months-old chicks inoculated buccal mucous membrane, 
after scarification, with a culture, show no ill-effects to date 10/9/12. 
(e) The subcutaneous injection of an emulsion in bouillon of several 
nodules collected from the lungs of ostrich (No. 6) to the rabbit, guinea- 
pig, and ostrich. 
(/) The subcutaneous injection of an emulsion in bouillon of portion 
of fungus growth collected from the hollow cavities of bones of the 
sternum of ostrich No. 6. 
(g) The ingestion of portion of contents of small intestines of 
ostrich No. 6 in which were included nodules. 
The negative results obtained from subcutaneous injection of nodules 
and ingestion of nodules indicate that infection takes place through the 
agency of spores only. 
Summary of Cultural Experiments. 
In several instances medium (potato) was inoculated for the purpose 
of ascertaining whether Aspergillus fumigatus could be recovered from 
affected organs. In the following the results were positive : — 
(a) Inoculation of medium with nodules and scrapings of organs show- 
ing nodules, e.g., lung, liver. 
(h) Inoculation of medium with scrapings of lungs which on post- 
mortem showed pathological anatomical lesions of hypersemia and oedema. 
(c) Inoculation of medium with scrapings of liver which on post- 
mortem appeared normal (1 experiment). 
