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Transactions of the Royal Society of South Africa. 
In the following instances negative results were obtained : — 
{a) Inoculation of medium with scrapings of lungs which on post- 
mortem showed hyperaemia and oedema (1 experiment). 
(h) Inoculation of medium with scrapings of liver which on post- 
mortem presented no pathological appearances (1 experiment). 
(c) Inoculation of medium with scrapings from kidneys of three 
ostriches which on post-mortem examination were found to be affected 
but in which the kidneys were apparently healthy. 
PATHOLOGY. 
Summary of Pathological Appearances and Seat of Lesions. 
As the result of intra-venous injections of a culture, the most constant 
seat of lesions was found to be in the liver and lungs. In the former they 
appear in the form of isolated, disseminated, miliary opaque nodules 
averaging -2 mm. in diameter in size. In the latter they also occur as 
isolated nodules from -2 to -5 mm. in diameter, white or yellowish in 
colour, firm in consistency. 
Hyperaemia of the small and large intestines and vermiform appendices 
was observed in two cases. The kidneys presented no pathological 
changes. 
Infection by inhalation produced a hyperaemic and oedematous 
condition of the lungs, with, in some cases, the appearance of nodules in 
the lung parenchyma, walls of the thoracic air-sacs and peritoneum. 
The ingestion of cultures resulted in no marked pathological changes 
in the intestinal tract, but lung lesions were noted. 
Inoculation of the buccal mucous membrane in young chicks resulted 
in one instance in lung and intestinal lesions with an exudate into the 
peritoneal cavities, and in another nodules were detected in the lungs. 
Introduction of spores direct into the trachea caused hyperaemia and 
oedema of the lungs and disseminated nodules, and nodules in the walls 
of the thoracic air-sacs and on the peritoneum, distension of the intestines 
with gas and an emphysematous condition of the subcutaneous tissue of 
the body. 
The most constant seat of lesions in experimentally produced cases 
was found to be in the lungs (Pneumomycosis). 
Microscopical Appearances. 
Portions of the liver and lungs collected from a 9-months-old chick 
which had died from intravenous inoculation of a culture were fixed in 
10 per cent, formalin and embedded in paraffin. Sections were stained 
