longitudinally along the epicardial coronaries and 
into the penetrating branches). The premise of the 
studies is that accelerated transplant atherosclero- 
sis represents an interaction of the recipient's im- 
mune cells with the growth factor systems govern- 
ing proliferation of vascular smooth muscle cells. A 
rat model of accelerated transplant atherosclerosis 
has been established, and probes for a number of 
rat growth factors and growth factor receptors have 
been cloned. Immunohistochemistry and in situ 
hybridization techniques are being used to identify 
the cells (T cells, B cells, monocytes) and molecules 
PUBLICATIONS 
(grov^^h factors, cytokines, and their receptors) that 
are present during the genesis of accelerated ath- 
erosclerosis in the rat model. Spatial and temporal 
correlations with cell proliferation (identified by 
immunohistochemical staining for cyclin) in the 
artery wall will be made. Cell culture models of 
the interactions of lymphocytes with endothelial 
and vascular smooth muscle cells are being de- 
veloped. 
Dr. Coughlin is also Assistant Professor of Medi- 
cine at the University of California at San Francisco. 
Articles 
Coughlin, S.R., Escobedo, J.A., and Williams, L.T. 1989. Role of phosphatidylinositol kinase in PDGF receptor 
signal transduction. Science 243:1191-1194. 
Williams, L.T., Escobedo, J.A., Keating, M.T., and Coughlin, S.R. 1988. Signal transduction by the platelet- 
derived growth factor receptor. Cold Spring Harb Symp Quant Biol 53:455-465. 
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