single band migrating with = 130,000 was evi- 
dent in the cells transfected with the cyclase vector. 
This band was not seen in the presence of an excess 
of nonradioactive ANP or in cells transfected with 
vector alone. The mobility of the radioactive band 
coincides with that previously reported for the high- 
molecular-weight ANP receptor. Thus the guanylate 
cyclase cDNA encodes a protein possessing both 
guanylate cyclase and ANP-binding activities. 
III. Primary Structure of the Resact Precursor. 
Previous studies had shown that the speract pre- 
cursor contained multiple copies of speract and 
speract-like peptides. In addition, other potential 
PUBLICATIONS 
peptides of unknown function were present in the 
precursor polypeptide. The cDNA encoding resact 
was isolated and sequenced to determine whether 
the precursor of an egg peptide from another sea 
urchin species would contain conserved sequences; 
this allowed probing for such egg peptides across 
the species. Unlike the speract precursor, the resact 
precursor contained only a single peptide corre- 
sponding to resact, and there were no significant 
similarities between the speract and resact pre- 
cursors. 
Dr. Garbers is also Professor of Pharmacology 
and of Molecular Physiology and Biophysics at the 
Vanderbilt University School of Medicine. 
Books and Chapters of Books 
Garbers, D.L. 1989. The regulation of spermatozoan function by the egg. In The Molecular Biology of Fertil- 
ization (Schatten, H., and Schatten, G., Eds.). New York: Academic, pp 3-19. 
Articles 
Bentley J.K., Khatra, A.S., and Garbers, D.L. 1988. Receptor-mediated activation of detergent-solubilized 
guanylate cyclase. Biol Reprod 39:639-647. 
Chinkers, M., Garbers, D.L., Chang, M.-S., Lowe, D.G., Chin, H., Goeddel, D.V, and Schulz, S. 1989. A mem- 
brane form of guanylate cyclase is an atrial natriuretic peptide receptor. Nature 338:78-83. 
Dangott, L.J., Jordan, J.E., Bellet, R.A., and Garbers, D.L. 1989. Cloning of the mRNA for the protein that 
crosslinks the egg peptide to speract. Proc Natl Acad Sci USA 86:2128-2132. 
Domino, S.E., Bocckino, S.B., and Garbers, D.L. 1989- Activation of phospholipase D by the fucose-sulfate 
glycoconjugate that induces an acrosome reaction in spermatozoa. J Biol Chem 264:9412-9419 . 
Garbers, D.L. 1988. Signal/transduction mechanisms of sea urchin spermatozoa. Biochemistry 1:120-126. 
Garbers, D.L. 1989. Guanylate cyclase, a cell surface receptor. J Biol Chem 264:9103-9106. 
Garbers, D.L. 1989. Molecular basis of fertilization. Annu Rev Biochem 58:719-742. 
Garbers, D.L. 1989. Molecular basis of signalling in the spermatozoon. J Androl 10:99-107. 
Garbers, D.L., Lowe, D.G., Dangott, L.J., Chinkers, M., Thorpe, D.S., Bentley, J.K., Ramarao, C.S., Goeddel, 
D.V, and Singh, S. 1988. The membrane form of guanylate cyclase. Cold Spring Harb Symp Quant Biol 
53:993-1003. 
Lowe, D.G., Chang, M.-S., Hellmiss, R., Chen, E., Singh, S., Garbers, D.L. , and Goeddel, D.V 1989. Human 
atrial natriuretic peptide receptor defines a new paradigm for second messenger signal transduction. 
£MfiO/ 8: 1377-1384. 
Schulz, S., Chinkers, M., and Garbers, D.L. 1989. The guanylate cyclase/receptor family of proteins. FASEBJ 
3:2026-2035. 
Thorpe, D.S., and Garbers, D.L. 1989- The membrane form of guanylate cyclase. Homology with a subunit of 
the cytoplasmic form of the enzyme. J Biol Chem 264:6545-6549 . 
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