ceptor, LDL receptor, parathyroid hormone, adeno- 
sine deaminase, and (3-galactosidase from Es- 
cherichia coli (i.e., Iac2?). Transplantation of genet- 
ically modified endothelial cells was demonstrated 
in a canine model previously used to study throm- 
bosis. Recombinant retroviruses were used to trans- 
duce the lacZ gene into canine endothelial cells. 
Prosthetic vascular grafts seeded with genetically 
modified cells were implanted as carotid interposi- 
tion grafts into the dogs from which the original 
cells were harvested. Analysis of the grafts 5 weeks 
PUBLICATIONS 
after implantation revealed genetically modified en- 
dothelial cells lining their luminal surfaces. This 
technology provides an opportunity to study the ef- 
fects of recombinant gene expression on endothe- 
lial cell function in vivo and has potential applica- 
tions to the treatment of vascular disease and the 
design of new drug delivery systems. 
Dr. Wilson is also Assistant Professor of Internal 
Medicine and of Biological Chemistry at the Univer- 
sity of Michigan Medical School. 
Books and Chapters of Books 
Chowdhury J.R., Chowdhury N.R., Demetriou, A.A., and Wilson, J.M. 1989. Use of microbeads for cell trans- 
plantation. \n Advanced Research on Animal Cell Technology (Miller, A.O.A., Ed.). Dordrecht: Kluwer Aca- 
demic, pp 315-327. 
Articles 
Davidson, B.L., Chin, S.-J., Wilson, J.M. , Kelley WN., and Palella, T.D. 1988. Hypoxanthine-guanine phos- 
phoribosyl transferase: genetic evidence for identical mutations in two partially deficient subjects. / Clin 
Invest 82:2164-2167. 
Wilson, J.M. , Birinyi, L.K., Salomon, R.N., Libby P, Callow, A.D., and Mulligan, R.C. 1989. Implantation of vas- 
cular grafts lined with genetically modified endothelial cells. Science 244:1344-1346. 
Wilson, J.M. , Johnston, D.E., Jefferson, D.M., and Mulligan, R.C. 1988. Correction of the genetic defect in he- 
patocytes from the Watanabe heritable hyperlipidemic rabbit. Proc Natl Acad Sci USA 85:4421-4425. 
132 
