III. Human Monocyte-derived FAF in Wound Heal- 
ing and Fibrosis. 
The range of effects of highly purified native FAF 
on human dermal fibroblasts has been delineated 
by Dr. Goetzl and Dr. Christoph W Turck in a vari- 
ety of assay systems, in comparison with other 
growth factors. At the different respective concen- 
trations that elicited the same levels of fibroblast 
proliferation, transforming growth factor-P (TGF-P), 
interleukin-1 (IL-1), acidic fibroblast growth factor 
(aFGF), and FAF all stimulated fibroblasts to gener- 
ate similar quantities of prostaglandin and pro- 
teoglycans. The magnitude of the stimulation of 
proteoglycan synthesis varied most widely among 
the factors, and TGF-p had the least effect. In con- 
trast, the production of coUagen evoked by FAF was 
found to be only minimal, compared with that in- 
duced by IL-1 and aFGF. Thus the functional defini- 
tion of FAF as a selective fibroblast growth stimulus, 
with no effect on endothelial or smooth muscle 
cells, has been extended to a distinctive profile of 
effects on fibroblasts. 
PUBLICATIONS 
Knowledge of some of the polypeptide structure 
of FAF has permitted studies of expression in alveo- 
lar macrophages (AMs) of patients with diverse 
inflammatory and fibrosing diseases of the lung. 
AM-derived cDNAs encoding messages for FAF and 
10 other growth factors were amplified by polymer- 
ase chain reaction (PGR) with a pair of 3' and 5' 
primers for each factor. Whereas AMs from three 
subjects with no lung disease expressed messages 
for IL-ip, TGF-p, and occasionally insulin-like 
growth factor 1 (IGF-1), these and epidermal 
growth factor (EGF), TGF-a, IL-la, and/or platelet- 
derived growth factor (PDGF) were found in three 
patients with inflammatory and three patients with 
fibrosing lung diseases. Analyses of genetic mes- 
sages in immune effector cells thus may provide 
useful prognostic information in some multigenic 
diseases. 
Dr. Goetzl is also Robert L. Kroc Professor of 
Rheumatic Diseases and Professor of Medicine and 
of Microbiology and Immunology at the University 
of California at San Francisco. 
Books and Chapters of Books 
Goetzl, E.J., Finch, R.J., Peterson, K.E., Turck, C.W, and Sreedharan, S.P 1989. Mast cell and basophil media- 
tion of lymphocyte functions. In Mast Cell and Basophil Differentiation and Function in Health and Dis- 
ease (Galli, S.J., and Austen, K.F., Eds.). New York: Raven, pp 247-254. 
Goetzl, E.J., and Goldstein, I.M. 1989. Arachidonic acid metabolites. In Arthritis and Allied Conditions 
(McCarty, J.D., Ed.). Philadelphia, PA: Lea & Febiger, pp 409-425. 
Goetzl, E.J., and Goldstein, I.M. 1989. Granulocytes. In Textbook of Rheumatology (Kelley, WN., Harris, E.D., 
Jr., Ruddy, S., and Sledge, C.B., Eds.). Philadelphia, PA: Saunders, 3rd ed, pp 322-345. 
Goetzl, E.J., Sreedharan, S.P, and Harkonen, WS. 1988. Pathogenetic roles of neuroimmunological media- 
tors. In Immunology and Allergy Clinics of North America (Goldstein, R.A., Ed.). Philadelphia, PA: 
Saunders, pp 183-200. 
Sreedharan, S.P, Peterson, K.E., Kodama, K.T, Finch, R.J., Taraboulos, A., Serwonska, M.H., and Goetzl, E.J. 
1989. Generation and recognition of neuropeptide mediators of cellular communication in immunity and 
hypersensitivity. In Neuroimmune Networks: Physiology and Diseases (Spector, N.H., and Goetzl, E.J., 
Eds.). New York: Liss, pp 113-118. 
Articles 
Atkins, PC, Valenzano, M., Goetzl, E.J., Ratnoff, WD., Graziano, F.M., and Zweiman, B. 1989. Identification of 
leukotriene B4 as the neutrophil chemotactic factor released by antigen challenge from passively sensi- 
tized guinea pig lungs. J Allergy Clin Immunol 83:136-143. 
Baud, L., Healy A., Cragoe, E.J. , Jr., Goetzl, E.J., and Koo, C.H. 1988. Leukotriene D^-induced increases in the 
cytoplasmic pH of human myelocytic leukocytes. J Cell Physiol 136:355-360. 
Burrall, B.A., Cheung, M., Chiu, A., and Goetzl, E.J. 1988. Enzymatic properties of the 15-lipoxygenase of 
human cultured keratinocytes. J Invest Dermatol 191:294-297. 
Continued 
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