lively reconstitute B lineage function in such mice, 
as exemplified by production of serum IgM. Co-in- 
oculation of T cells with the cultured B cell progen- 
itor lines induced class switching and the expres- 
sion of multiple immunoglobulin isotypes. These 
studies help to define the earliest known cell type 
for the B cell lineage and should be valuable for the 
PUBLICATIONS 
definition of developmental controls for the B cell 
lineage and the relationship of B cell progenitors to 
more primitive stem cells. 
Dr. Witte is also Professor of Microbiology and 
Member of the Molecular Biology Institute at the 
University of California at Los Angeles. 
Books and Chapters of Books 
Clark, S.S., Najfeld, V, Dow, L., Crist, WM., Champlin, R., and Witte, O.N. 1988. Molecular pathogenesis of 
BCR-ABL oncogene expression in Ph"*" leukemia. In Molecular Diagnosis of Human Cancer (Greaves, M., 
Ed.). Cold Spring Harbor, NY: Cold Spring Harbor, vol 7, pp 15-20. 
Denis, K.A. , and Witte, O.N. 1988. Long term lymphoid cultures in the study of B cell differentiation. In Im- 
munoglobulin Genes (Alt, F.W, Honjo, T., and Rabbitts, T.H., Eds.). London: Academic, pp 45-59. 
Fathman, C.G., Fitch, F.W, Denis, K.A., and Witte, O.N. 1988. Critical techniques for the study of the immune 
system: long term culture of immunocompetent cells. In Fundamental Immunology (Paul, WE., Ed.). New 
York: Raven, 2nd ed, pp 803-815. 
Articles 
Clark, S.S., Crist, WM., and Witte, O.N. 1988. Molecular pathogenesis of Ph-positive leukemias. Annu Rev 
Med 40:115-122. 
Denis, K.A. , Timson, L.K., and Witte, O.N. 1989- Vp^ repertoire in progeny of long term lymphoid-cultured 
cells used to reconstitute immunodeficient mice. J Immunol 142:2981-2987. 
Denny C.T, Shah, N.R, Ogden, S., Willman, C, McConnell, T, Crist, W, Carroll, A., and Witte, O.N. 1989. Lo- 
calization of preferential sites of rearrangement within the BCR gene in Philadelphia chromosome-positive 
acute lymphoblastic leukemia. Proc Natl Acad Sci USA 86:4254-4258. 
Dow, L.W, Tachibana, N., Raimondi, S.C., Lauer, S.J., Clark, S.S., and Witte, O.N. 1989. Comparative biochemi- 
cal and cytogenetic studies of childhood acute lymphoblastic leukemia with the Philadelphia chromosome 
and other 22q 11 variants. Blood 73:1291-1297. 
Lugo, T.G., and Witte, O.N. 1989. The BCR/ABL oncogene transforms Rat-1 cells and cooperates with y-myc. 
Mol Cell Biol 9:1263-1270. 
Manz, J., Denis, K., Witte, O.N., Brinster, R., and Storb, U. 1988. Feedback inhibition of immunoglobulin gene 
rearrangement by membrane but not by secreted |jl heavy chains. J Exp Med 168:1363-1382. 
McLaughlin, J., Chianese, E., and Witte, O.N. 1989. Alternative forms of the BCR-ABL oncogene have quantita- 
tively different potencies for stimulation of immature lymphoid cells. Mol Cell Biol 9: 1866-1874. 
Pendergast, A.M., Clark, R., Kawasaki, E.S., McCormick, F.P, and Witte, O.N. 1989. Baculovirus expression of 
functional P210 BCR-ABL oncogene product. Oncogene 4:759-766. 
Ponzetto, C, Wadewitz, A.G., Pendergast, A.M., Witte, O.N. , and Wolgemuth, D.J. 1989- Vl^Q^'^^^ is detected 
in mouse male germ cells by an in vitro kinase assay and is associated with stage-specific phosphoproteins 
in haploid cells. Oncogene 4:685-690. 
Timmons, M.S., and Witte, O.N. 1989. Structural characterization of the BCR gene product. Oncogene 4:559- 
567. 
Young, J.C., and Witte, O.N. 1988. Selective transformation of primitive lymphoid cells by the BCR/ABL 
oncogene expressed in long-term lymphoid or myeloid cultures. Mol Cell Biol 8:4079-4087. 
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