sequence unique to this protein recognizes an —40 
kDa protein that is enriched in preparations of nu- 
clei from female Drosophila bodies. Several 
monoclonal antibodies that recognize xhe Drosoph- 
ila (3-subunit react strongly with Drosophila eyes, 
brain, and thoracic ganglion. In ovaries, these anti- 
bodies also react strongly with nuclei of nurse cells 
and follicle cells. 
Dr. Hurley and his colleagues continue to investi- 
gate the roles of G proteins in Drosophila, using a 
variety of methods, including transformation, v^ith 
G protein genes having dominant mutations, and 
production of G protein null mutants. The role of a 
PUBLICATIONS 
G protein in nuclei is of particular interest. Similar 
methods are also being used to study transducins 
in mammalian photoreceptors. The mouse rod 
transducin gene has been isolated, and its pro- 
moter is being characterized by testing its abUity to 
express p-galactosidase in rod cells of transgenic 
mice. Once expression in photoreceptors has been 
achieved, the effects of dominant mutations on the 
kinetics and sensitivity of phototransduction are to 
be determined. 
Dr. Hurley is also Assistant Professor of Biochem- 
istry at the University of Washington. 
Books and Chapters of Books 
Lerea, C, Raport, C., Dere, B., Bunt-Milam, A., and Hurley, J.B. 1988. Transducins in human rod and cone 
photoreceptors and their relationships to other G proteins. In Molecular Biology of the Eye: Genes, Vi- 
sion, and Ocular Disease. Proceedings of a National Eye Institute-UCLA Symposium, Santa Fe, New Mex- 
ico, February 6-12, 1988 (Piatogorsky, J., Shinohara, T, and Zelena, PS., Eds.). New York: Liss, pp 53-62. 
Articles 
Lerea, C.L., Bunt-Milam, A.H., and Hurley, J.B. 1989. a transducin is present in blue-, green-, and red-sensi- 
tive cone photoreceptors in the human retina. Neuron 3:367-376. 
Raport, C.J., Dere, B., and Hurley, J.B. 1989. Characterization of the mouse rod transducin a subunit gene. 
fBiolChem 264:7122-7128! 
Yarfitz, S., Provost, N.M., and Hurley, J.B. 1988. Cloning of a Drosophila melanogaster guanine nucleotide 
regulatory protein (3-subunit gene and characterization of its expression during development. Proc Natl 
AcadSci f/5A 85:7134-7138. 
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