veloped a two-color, multiple fluorescent label, 
DNA in situ hybridization scheme. One DNA is la- 
beled with a biotin probe, while the other DNA is 
labeled with a digoxigenin probe. Thus an internal 
DNA control is present during the molecular/struc- 
tural alignment in polytene band/interbands. These 
tools are also used for in situ hybridization of mini- 
Dr. Sedat is also Professor of Biochemistry and 
Biophysics at the University of California at San 
Francisco. 
mally perturbed polytene chromosomes, so that 
three-dimensional localization questions can be 
asked. 
PUBLICATIONS 
Books and Chapters of Books 
Hochstrasser, M., Mathog, D., Gruenbaum, Y, Saumweber, H., and Sedat, J.W 1988. Three-dimensional orga- 
nization of interphase chromosome in polytene nuclei of Drosophila melanogaster. \n Architecture ofEu- 
karyotic Genes (Kahl, G., Ed.). Weinheim, FRG: VCH Verlagsgesellschaft MBH, p 473. 
Agard, D.A., Hiraoka, Y, Shaw, E, and Sedat, J.W 1989. Fluorescence microscopy in three dimensions. Me^/??- 
ods Cell Biol 30:353-377. 
Aikens, R.S., Agard, D.A. , and Sedat, J.W 1989. Solid-state imagers for microscopy. Methods Cell Biol 29:291- 
313. 
Mathog, D., and Sedat, J.W The three-dimensional organization of polytene nuclei in male Drosophila 
melanogaster with compound XFor ring X chromosomes. Genetics 121:293-311. 
Minden, J.S., Agard, D.A. , Sedat, J.W , and Alberts, B.M. 1989. Direct cell lineage analysis in Drosophila 
melanogaster by time-lapse, three-dimensional optical microscopy of living embryos. / Cell Biol 109:505- 
516. 
Rykowski, M.C., Parmalee, S.J., Agard, D.A., and Sedat, J.W 1988. Precise determination of the molecular lim- 
its of a polytene chromosome band: regulatory sequences for the Notch gene are in the interband. Cell 
54:461-472. 
Shaw, EJ., Agard, D.A., Hiraoka, Y, and Sedat, J.W 1989. Tilted view reconstruction in optical microscopy. 
Three-dimensional reconstruction of Drosophila melanogaster embryo nuclei. Biophys J 55:101-110. 
Articles 
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