216 
PULMONARY MODELS 
minutes ischemia time, you can have almost 100 
per cent long-term survival with xenon stu- 
dies. The technique can be used practically for 
large volume experiments. 
Chairman : Thank you very much Dr. Moss. 
I've seen Dr. Moss* technique, and it does repre- 
sent quite an advance. Are there any other 
questions ? 
J. MOOR-JANKOWSKI, New York University 
School of Medicine: What were the levels of 
heteroagglutinins in the serum of the donor 
animal, which of course would come from the 
interstitial tissue into the lung after the trans- 
plantation? 
Dr. Cook : Do you mean in the serum of the 
recipient animal or donor animal? We at- 
tempted to measure and wash out all the donor 
heteroagglutinins. And when we measured ag- 
glutination titers on the eluate from the washed 
out cat lung, we saw no agglutination activity. 
Dr. Moor-Jankowski : From the eluates 
from what? 
Dr. Cook: The eluates from the perfusion 
before the lung was hooked into the circuit. 
It's possible that there were interstitial anti- 
bodies we couldn't measure, but in our in vitro 
system for measuring heteroagglutinins we did 
not detect any, and we made a specific effort to 
wash them all out. 
Dr. Moor-Jankowski : But did you check the 
level before the transplantation? 
Dr. Cook : Yes, we checked the level as best 
we could by washing the organs and measuring 
the eluate. 
Dr. Moor-Jankowski : What I want to know 
is if you measured the titer and if you knew 
what it was before? 
Chairman : Yes, we did. In the serum it's ex- 
tremely high, and it varies from animal to 
animal. 
