230 
PULMONARY MODELS 
Table L — Experimental Protocol 
Experimental Procedure 
Number 
Group of Dogs Infusion Rate Duration Intervention 
I 3 Untreated Controls 
II 6 Intermittent 50 ml/kg/hr 25 min ea hr for 6 hrs 
III 6 Continual 100 mlAe/hr 4-6 hrs 
IV 5 Continual 100 ml/kg/hr 4-6 hrs furosemide (Lasix ( furosemide) , Hoechst) 
(0.8 mg/kg) every 2 hrs 
iologic parameters monitored included total and 
resistive respiratory work, hematocrit, urine 
volume, urine and serum osmolalities, arterial 
pH and blood gases, EKG, aortic and central 
venous pressure. The experimental design for 
collecting these data are presented in Figure 1. 
Biopsies of each of the lobes of both lungs 
w^ere obtained for microscopic examination. For 
light microscopy, samples of lung were fixed in 
glutaraldehyde or formalin. Paraffin sections 
were prepared in the standard manner. Slides 
were stained with hematoxylin-eosin, phospho- 
Pneumotachograph 
Surital |j 
Infusion 
Volunne 
Infusion 
Flow 
Pressure 
pH 
Hct 
PaOa 
PaCOa 
O2 Saturation 
Osmolality 
® 
Urine Volume 
Osmololity 
tungstic acid-hematoxylin, and Mallory's ana- 
line blue. 
For transmission electron microscopy, the 
lung tissue was placed in cold (4°C.) 3% 
glutaraldehyde in 0.1 M Sorenson's phosphate 
buffer (pH 7.4) and cut into millimeter-square 
cubes. The tissue was fixed at 4°C. in glutaral- 
dehyde solution for 24 hours, washed in 0.1 M 
Sorenson's phosphate buffer, post-fixed in 2% 
osmium tetroxide in Sorenson's phosphate buf- 
fer, rinsed twice in cold distilled water, and 
dehydrated through a graded series of ethyl 
Work 
( Total/ Breath and Resistive/Breath) 
Compliance 
Minute Volume 
Total Work/L and 
/min 
Resistive Work/L 
and 
/min 
I * ♦ 
6 Channel Recorder 
PA Wedge Pressure 
Venous Pressure 
Arterial Pressure 
EKG 
Heart Rate 
Figure 1. — Experimental Design. 
