E. B. BLACK AND J. M. STENGLE 
457 
ANTIBODY 
TITER 
256 
64 
I 6 
4 
0 
.20 
^^JO O // O O 
WBC 
xlO^ 
DONATED RBC 
SURVIVING 50 
20 ML 
A. RBC 
DAYS 
- 256 
- 64 
SERUM 
16 COMPLEMENT 
TITER 
0--0 
PLATELETS 
X 10^ 
O- -o 
PLASMA 
HGB 
M6M % 
0--0 
Figure 8. — The principal hematologic and serologic changes following infusion of type Ai red cells to a sensitized 
A negative dog." 
culation, probably as a result of an immune 
adherence reaction with incompatible erythro- 
cytes in the case of administration of incompat- 
ible cells. Platelet sequestration probably re- 
sults from a similar sequence of events in the 
case of administration of anti-A-antisera to an 
A positive dog. In addition to the thrombocyto- 
penia, Friesen, Harsha, and McCroskey^^ have 
reported fibrinolytic activity and a heparin-like 
activity in the plasma of recipient dogs resem- 
bling the syndrome of disseminated intravascu- 
lar coagulation. 
Clinically significant transfusion reactions 
involving other canine blood types are rare. Ad- 
ministration of group C blood to a sensitized C 
negative dog can cause a severe reaction ; how- 
ever, since 98% of dogs are C positive, C incom- 
patibility reactions are of little practical impor- 
tance. Likewise, F negative dogs are a rarity. B, 
D, E, and G groups are only weakly antigenic. 
and clinically recognizable transfusion reac- 
tions involving them are infrequent. Hemolytic 
transfusion reactions have not been described 
in sheep and those in sensitized cattle run a 
course similar to those in dogs. 
Hemolytic disease of newborn puppies has 
been seen when type Ai puppies feed on the 
clostrum of dams previously sensitized to Ai 
erythrocytes by transfusion.'*'^ Anti Ai- anti- 
bodies are adsorbed through the gastroin- 
testinal mucosa into the circulation of the new- 
born pup resulting in a hemolytic anemia often 
so severe as to be fatal during the first few days 
of life. After the first day of life, the gastroin- 
testinal mucosa of the newborn dog apparently 
loses its ability to absorb active anti-A-anti- 
body, and A positive puppies which have been 
fed artificially or by a non-sensitized bitch dur- 
ing the first 24 hours may be returned to their 
dam. 
