466 
HEMATOLOGY 
NORMAL RABBIT PLASMA 
J L 
0 t 1 
(a) 
TIME (Hrs.) 
2 
(b) 
Figure 2. — a. Factor VIII activities of 4 spleen and liver perfusions after addition of 5 ml fresh normal rabbit 
plasma as indicated by the arrow, b. Factor IX activities of the same experiments shown in 2a. 
greater factor VIII activity at 30 min in control 
splenic perfusions as compared with hepatic 
perfusions (Figure la), the addition of normal 
plasma to splenic perfusates (Figure 2a) re- 
sulted in slightly higher factor VIII activity in 
the spleen than in the liver (p = < 0.05) . In ad- 
dition, factor IX activities immediately after 
addition of normal plasma were equivalent in 
perfusates from both organs, a result which 
would be predicted on the basis of similar control 
levels of factor IX at 30 min (Figure lb) . 
Figure 3 presents data following addition of 
plasma from the rabbits used in the experi- 
ments shown in Figure 2, but after these same 
animals had been treated with warfarin. The 
plasma obtained from these warfarin-treated 
rabbits was factor VII and IX deficient. It can 
be seen that factor VIII production ceased in 
perfused livers and spleens (Figure 3a) while 
factor IX production continued (Figure 3b). 
Hepatic factor IX production in these experi- 
ments appeared similar to that observed in con- 
trol perfusions without additives (Figure lb). 
Studies following addition of normal human 
plasma are shown in Figure 4. Perfusate coagu- 
lation activities were increased after addition 
of normal plasma, but it can be seen in Figure 
4a that neither fresh plasma nor fresh-frozen 
plasma was capable of raising the perfusate 
factor VIII activities sufficiently to inhibit fur- 
ther production. These findings are not surpris- 
ing if one considers the relatively high factor 
VIII levels of most animal species as compared 
with those in man. It was determined in six 
paired experiments that rabbit plasma was 2.5 
(range 1.9 to 3.0) times more active with re- 
spect to factor VIII activity but only 1.1 (range 
0.9 to 1.5) times more active in factor IX activ- 
ity than human plasma. Fresh-frozen normal 
human plasma contained relatively less factor 
VIII (50-75% ) than the same samples that had 
not been frozen. The generation of splenic fac- 
tor VIII activity with frozen plasma was more 
rapid between 30 min and 3 hr than that ob- 
