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DISCUSSION 
Franklin G. Robinson, Cutter Lab., Berke- 
ley, California: What dosage of Pluronic was 
used? 
Dr. Shepard: This was the older Pluronic 
obtained as flakes from the Wyandotte Corpora- 
tion. It was dissolved in distilled water at a 
concentration of 3.3 grams per cent, and the 
contents were diluted in the oxygenator so that 
the final concentration was .6 of a milligram per 
milliliter, which is the same concentration re- 
ported in the literature. As you know, there's a 
lot of literature about the use of Pluronic. Dr. 
Lester Bryant from Kentucky, who is attending 
this conference, has published a paper about its 
clinical use. 
Dr. Robinson: Is that with or without 
Ironal? 
Dr. Shepard: The material was furnished 
simply as Pluronic F-68. A preservative may 
have been present. If so, no statement about its 
presence was made to us by the manufacturer. 
Jeffrey L. Peters, University of Utah, Salt 
Lake City, Utah: How were the red cells 
handled during this twenty-hour period ? 
Dr. Shepard : The red cells during the period 
of oxygenation of the plasma were refrigerated 
in plastic bags. They had been spun down and 
maintained at 4°C. 
