718 
PHARMACOLOGY 
Table II. — Species Differences in Plasma Protein 
Binding of Salicylate'' 
Plasma Protein 
Percent Bound By 
SpGcies 
Concentration 
g/100 ml. 
% 
Human 
6.3 
85 
8.0 
85 
7.9 
38 
DoK 
6.7 
70 
6.9 
66 
7.2 
63 
Goat 
6.4 
64 
6.7 
58 
6.6 
63 
Swine 
6.3 
64 
7.8 
68 
7.9 
72 
Pony 
6.8 
54 
7.0 
52 
6.7 
57 
Cat 
6.5 
64 
7.7 
64 
7.9 
60 
.50mg/kg, Dose Level 
Col 
" Three different individuals of each species were studied. 
fluorometric procedure was developed to meas- 
ure phenol in biological materials. This method 
was specific and sensitive. 
Plasma disappearance and urinary excretion 
of intravenously administered phenol were 
studied in dogs, cats, swine and goats. Cats 
were acutely poisoned by dosages of 50 mg/kg, 
whereas dogs, pigs, and goats suffered sublethal 
intoxication from doses of 100 mg/kg. Plasma 
disappearance of injected phenol was most 
rapid in goats, slower in pigs and dogs, and 
slowest in cats (Figure 3, and Tables III-VI). 
The time required for most of the injected 
phenol to be in the conjugated form in the 
plasma was directly proportional to the rate of 
plasma disappearance and inversely related to 
the dose given. 
Table III. — The Kinetic Constants for Plasma 
Disappearance of Phenol in Dogs 
Time. Hours 
Figure 3. — Disappearance of injected free "C-phenol 
from the plasma of dogs, cats, swine and goats. The 
curves were adjusted slightly to a similar concentra- 
tion at time zero. 
The apparent specific volumes of distribution 
of free phenol in dogs, pigs and goats indicated 
extensive tissue binding of phenol. Such evi- 
dence for tissue binding was not observed in 
Table V. — The Kinetic Constants for Plasma 
Disappearance of Phenol in Pigs 
Dose 
Co 
TV2 
Kd 
Vd 
Dose 
Co 
T% 
Kd 
V'd 
mg.AK- 
10 
60 
100 
dpm X lOVml. 
4.2 
5.9 
9.3 
hrs. 
1.36 
2.E6 
1.70 
hr.-i 
0.61 
0.27 
0.41 
L./kg. 
0.41 
1.69 
2.08 
mg./kg. 
10 
50 
100 
dpm X lOVml. 
0.8 
6.6 
15.8 
hrs. 
0.84 
0.54 
0.48 
hr.-i 
0.83 
1.28 
1.44 
L.Ak. 
2.40 
1.53 
1.72 
Table IV. — The Kinetic Constants for Plasma Table VI. — The Kinetic Constants for Plasma 
Disappearance of Phenol in Cats Disappearance of Phenol in Goats 
Dose 
Co 
TV2 
Kd 
V'd 
Dose 
Co 
T% 
Kd 
V'd 
me-/ke- 
dpm X lOVml. 
hrs. 
hr.-i 
L./kg. 
mg.Ag. 
dpm X lO'/ml. 
hrs. 
hr.-i 
L./kg 
10 
3.9 
1.22 
0.57 
0.47 
10 
2.4 
0.44 
1.68 
0.79 
30 
9.9 
1.67 
0.42 
0.19 
50 
8.8 
0.48 
1.43 
1.09 
60 
8.6 
7.00 
0.10 
0.19 
100 
14.8 
0.51 
1.36 
1.28 
