914 
GENETIC AND BIOLOGICAL EFFECTS OF RADIATION 
PLUTINA, 16 watt ^^^Pu 
RES- 24, 3308 
RES- 24, 3403 
RES-24, 4437 
WOLF, 24 watt "^Pu 
RES-24, 4172 
LITTER 1, Pup 7 
LITTER 1, Pup 9 
RES-24, 5453 
RES-24, 5932 
RES-50, BABOON 761-70 
RES- 50, BABOON 143-71 
RES-50, CHIMPANZEE 191-71 
LITTER 3, Pup 1 
2 
3 
4 
5 
6 
7 
July 
1967 
• Normal Chromosomal Morphology 
■ Changes in Chromosomal Morphology 
A Failure of Culture 
July 
1968 
July 
1969 
YEAR 
July 
1970 
July 
1971 
Figure 3. — Summary of chromosomal morphology data from radioisotope- 
bearing animals and their offspring. 
implantation, cultures from four dogs (Plutina, 
Wolf, RES #3308 and 4172) showed chromatid 
gaps and chromosomal breaks, gaps, fragmen- 
tation and rings (Figures 4 and 5). Later cul- 
tures, however, showed normal metaphase 
figures and normal karyotypes of these subjects. 
The rest of the anim&,ls, including the offspring, 
showed normal chromosomal morphology and 
karyotyping throughout the study (Figure 6). 
DISCUSSION 
Kozinets^ reviewed the literature on the ef- 
fects of ionizing radiation on human lympho- 
poiesis and lymphocytes and concluded that the 
absolute number of lymphocytes in the blood 
is a sensitive "biological" indicator. Studies of 
the hematologic effects of continuous exposure 
to radiation at dose rates ranging from 3 to 10 
rad/day have revealed that the lymphocytes 
