N. P. DEPASQUALE AND GEORGE E. BURGH 
949 
COXSACKIEVIRUS B4 VALVULITIS 
(Aortic Valve) 
CYNOMOLGUS MONKEY 
Figure 7. — Photomicrograph of a verrucous lesion of 
the aortic valve of a cynomolgus monkey inoculated 
with coxsackievirus Bi 74 days before autopsy (Am. 
Heart J. 71:678, 1966). 
mice infected with coxsackie virus B4. Science 
151:447, 1966. 
2. DePasquale, N. p., Burch, G. E., Sun, S. C, 
Hale, A. R., and Mobabgab, W. J. Experimental 
coxsackie virus B* valvulitis in cynomolgus mon- 
keys. Am. Heart J. 71:678, 1966. 
3. Kibrick, S., and Benirschke, K. Acute aseptic 
myocarditis and meningoencephalitis in the new- 
born child infected with coxsackie virus group B, 
type 3. New England J. Med. 255:883, 1956. 
Figure 8. — Localization of coxsackievirus Bj antigen 
in the mitral valve by direct immunofluorescence 
(x 320) (Am. Heart J. 71:678, 1966). 
4. Cherry, W. B., et al. Fluorescent Antibody Tech- 
nics in The Diagnosis of Communicable Diseases. 
Publication 729, U. S. Public Health Service, 1960. 
5. Pearce, J. M. Cardiac lesions in rabbits produced 
by a filterable virus (Virus III). Arch. Path. 
28:827, 1939. 
6. Pearce, J. M. Susceptibility of the heart of the 
rabbit to specific infection in viral diseases. Arch. 
Path. 34:319, 1942. 
7. Pearce, J. M. Heart disease and filterable viruses. 
Circulation 21:448, 1960. 
8. KiLHAM, L., Mason, P., and Davies, J. N. P. 
Host-virus relation in encephalomyocarditis (EMC) 
virus infections. II. Myocarditis in mongooses. Am. 
J. Trop. Med. 5:655, 1956. 
9. Lou, T., Wenner, H. a., and Kamitsuka, P. S. 
Experimental infections with coxsackieviruses. II. 
Myocarditis in cynomolgus monkeys infected with 
Bi virus. Archiv. forsch. Virusforschung 10:451, 
1960. 
10. Sun, S. C, Sohal, R. S., Burch, G. E., Chu, K. C, 
and COLCOLOUGH, H. L. Coxsackie virus B, pan- 
carditis in cynomolgus monkeys resembling rheu- 
matic heart lesions. Brit. J. Exper. Path. 48:655, 
1967. 
11. Burch, G. E., Sun, S. C, Colcolough, H. L., 
Sohal, R. S., and DePasquale, N. P. Coxsackie B 
viral myocarditis and valvulitis identified in routine 
autopsy specimens by immunofluorescent tech- 
niques. Am. Heart J. 74:13, 1967. 
DISCUSSION 
Morris Pollard, University of Notre Dame : 
It seems that you have been able to detect anti- 
gen in these tissues for long periods of time 
after the initial inoculation. Either there is a 
virus there which has persisted, in perhaps an 
integrated form, or else your fluorescein type 
antiserum is not too specific. The solution to 
this would be, first of all, to isolate and recover 
the virus from these tissues, which would be the 
definitive step. A second aspect of this very in- 
teresting and, I think, important presentation is 
the clarification of the age of the inoculated ani- 
mals because we do know that there is an age 
susceptibility to coxsackie infection which re- 
lates to the lethal effect of the virus. 
Dr. DePasquale: Yes. I didn't mention it, 
but we had numerous control animals in both 
groups. In the monkey group, we had five con- 
trols, and in the mouse group, 100 controls. 
We've infected, over the years, over a thousand 
mice, and we have not found any positive im- 
munofluorescence in the mice or in the monkeys. 
