W. C. DOLOWY AND L. J. SWANGO 
1029 
fied the cost of establishing the diagnostic- 
investigative animal laboratory. 
REFERENCES 
1. Mcpherson, C. Role of the National Institutes of 
Health in Support of Laboratory Animal Resources. 
JAVMA, 157:1959-1963, 1970. 
2. Henderson, J. D., Jr, and Giddens, W. E., Jr. Cal- 
cinosis Circumscripta Involving the Mammary 
Glands of a New Zealand White Rabbit. JAVMA, 
157-663-666, 1970. 
3. Henderson, J. D., Jr., and Giddens, W. E., Jr. 
Amyloidosis and Dermatomycosis in C57 BL Mice. 
JAVMA, 157:680-684, 1970. 
4. Giddens, W. E., Jr, and Dillingham, L. A. Fatal 
Asphyxiation in a Baboon (Papio anuhis) Caused 
by a Laryngeal Peduncular Fibroma. JAVMA, 
157:726-729, 1970. 
5. Giddens, W. E., Jr, and Dillingham, L. A. Pri- 
mary Tumors of the Lung in Nonhuman Primates : 
Literature Review and Report of Peripheral Car- 
cinoid Tumors of the Lung in a Rhesus Monkey 
(Macaca mulatta). Vet. Path. 8: (In Press) 1971. 
6. BiNN, L. N., Eddy, G. A., Lazar, E. C, Helms, J., 
and Murnane, T. Viruses Recovered from Labora- 
tory Dogs with Respiratory Disease. Proe. Soc. 
Exptl. Biol. (fe'Med. 126:140-145, 1967. 
7. SwANGO, L. J., Wooding, W. L., and Binn, L. N. 
A Comparison of the Pathogenesis and Antigenicity 
of Infectious Canine Hepatitis Virus and the 
A26/61 Virus Strain (Toronto). Am. J. Vet. Res. 
156:1687-1696, 1970. 
8. Giddens, W. E., Jr, Swango, L. J., Henderson, 
J. D., Jr, Lewis, R. A., Farner, D. S., Carlos, A., 
and DoLOWY, W. C. Canary Pox in Sparrows and 
Canaries (Fringillidae) and in Weavers (Plo- 
ceidae). Pathology and Host Specificity of the 
Virus. Vet. Path. 8: In Press, 1971. 
9. Glenn, B. L. Feline Porphyria: Comparative As- 
pects with Porphyria of other Domestic Animals 
and Man. In Animal Models for Biomedical Re- 
search IV, National Academy of Sciences, Wash- 
ington, D.C., pp. 135-148, 1971. 
10. Eisele, J. W. A Comparison of Fatty Liver of 
Pregnancy in Humans and Ketosis in Pregnant 
Ewes. Thesis for Master of Science degree. Uni- 
versity of Washington, 1972. 
APPENDIX -I 
Histologic Stains Frequently Used in the Histology 
Laboratory* 
H & E — Hematoxylin and Eosin 
H.B.F.P. — Hematoxylin-Basic Fuchsin-Picric Acid 
Luxol Fast Blue Myelin 
P.A.S. — Periodic Acid Schiffs (both standard and picro 
methyl blue techniques) 
Mallorys Phloxine Methylylene Blue 
Warthin-Starry Method 
Nuclear Fuelgen Stain 
Methyl Green 
Gram Stain 
Mucin Keratin 
Giemsa 
Mayers Mucicarmine 
Congo Red 
Oil Red 0 
Gomori Trichrome 
Gomori Methyl Silver 
Gomorl's Iron Reaction 
Glycogen 
Weigerts Fibrin 
Allochrome Procedure 
Prussian Blue 
A.F.B.— Acid Fast Bacilli 
Lendrum's Stain for Inclusion Bodies 
Hematoxylin — Shorr S3 Stain for Inclusion Bodies 
* Procedures, indications, and interpretations of these stains can 
be found in the following references: 
Manual of Histologic Staining Methods of the Armed Forees 
Institute of Pathology, 3rd ed. Ed. Lee G. Luna, HT(ASCP), 
McGraw-Hill Book Co.. New York, N.Y., 1968. 
Thompson, Samuel W., Selected Histochemical and Histopatho- 
logical Methods. Chai-les C. Thomas, Springfield, Illinois, 1966. 
APPENDIX- II 
Determinations and Methods Employed in the Clinical Pathology Laboratory Method 
Minimum amount 
of specimen to 
Test Method Type of Specimen be submitted* 
HEMATOLOGY: 
CBC _ Whole blood ^ 5-1 ml 
PCV Micro-hematocrit Whole blood (EDTA )..... - 25-.5 
WBC Coulter counter. do 25-.5 
RBC do do .25-.5 
Hgb — Cyanmethemoglobin colorimetric do 25-.5 
ESR. .. Westergren _. _ do 1-1.5 
Reticulocyte count Brilliant cresyl blue do .1 
Direct eosinophil count ... Pilot's diluting fluid do 2 
Direct platelet count Ammonium oxalate do 1 
MCV _ do Am't. sufficient for RBC, PCV, Hgb 
MCHC _ do Do. 
MCH _ _ _ do Do. 
Differential- Wright's stain do 1 
