1030 
ANIMAL RESOURCES 
APPENDIX — II (cont'd) 
Minimum amount 
of specimen to 
Test Method Type of Specimen be submitted* 
Urinalysis _ _ Urine 5 ml 
Ova & Parasites Direct, sugar flotation, MIP Feces Piece size of a pea 
Occult blood _ Orthotolidine _do-— Do. 
CHEMISTRY: 
SCOT - Karmen UV (Boehringer Mannheim) Serum — 2 ml clotted blood 
SGPT. - Wroblewski UV ( Boehringer Mannheim ) do Do. 
LDH.„ - Wroblewski-La Due colorimetric (BM) do 1-1.5 ml clotted blood 
Alkaline Bessey-Lowry colorimetric (BM) do 1.5-2.0 clotted blood 
Phosphatase 
Total protein Total solids meter _ _do 1 ml clotted blood 
Albumen Electrophoresis — do Do. 
Globulin do _ do _ — Do. 
Cholesterol Liebermann-Watson colorimetric (BM) do Do. 
Creatinine - Jaffe reaction colorimetric (BM) do — __ — 5 ml clotted blood 
Amylase. Smogyi visual (Sigma) do - — Do. 
Lipase CherryCrandall titrimetric (Sigma) do — 2 ml clotted blood 
Glucose Enzamatic with Smogyi filtrate (Glu- 1.5-2.0 ml blood 
costat-Worthington ) colorimetric Whole blood,** serum, plasma 5 whole blood 
BUN Modified Berthelot reaction 1 ml cl. blood 
colorimetric (Hyland) Whole blood,*** serum, plasma.— .25 whole blood 
Sodium Flame photometry Serum 2 ml cl. blood 
Potassium do _ do — Do. 
Chloride.. Schales and Schales (Sigma) titrimetric... do _. 1.5-2 ml cl. blood 
MICRO METHODS: 
Total protein _ Kingsley (Biuret) (Beckman) Gornall, 
Bradawill, David colorimetric do .25-.5 clotted blood 
A:G ratio do_ _ do — Do. 
Cholesterol Zak (Beckman) colorimetric. do _ - Do. 
Glucose Keston and Teller (Beckman) 
enzamatic — colorimetric do Do. 
Phosphorus Fiske and Subbarrow (Beckman) -do - Do. 
SCOT _ Karmen (Sigma) do — Do. 
SGPT do... do - Do. 
Bilirubin (total) Malloy and Evelyn (Beckman) 
colorimetric _ do Do. 
Bilirubin direct _do '. do - Do. 
Bilirubin indirect do do Do. 
Sodium and Potassium Flame photometry _ do — -5-1.5 clotted blood 
•Additional sample should be submitted if possible so that tests can be run in duplicate or dilutions made if necessary. 
♦♦Suitable anticoagulants — oxalate, EDTA, citrate, heparin. 
***Suitable anticoagulants — any common anticoagulant except ammonium oxalate. 
Note: Procedures for these methods are listed in text books and manuals of Clinical Pathology and Clinical Biochemistry. 
DISCUSSION 
F. Geller, Albert Einstein, New York: Is 
there a fee for this service ? 
Dr. Dolowy : No. 
C. B. Thayer, University of Iowa, Iowa: Bill, 
it seems to me that you might well charge a fee 
for laboratory work done on the planned proj- 
ects. For biological work, they get money in 
grants and this sort of thing, but I know it is 
not enough. 
Dr. Dolowy : It is conceivable that you could 
support one or maybe two technicians on say 
$15,000 or $20,000 a year this way, but I doubt 
whether the average investigator would be able 
to pay even those costs for which we currently 
receive most of the NIH money. For instance, 
we're getting $70,000 to $80,000 per year, about 
600 reports are going out per year. How much 
is that? A hundred, a hundred and something 
dollars per report. So if a man brings in a sick 
mouse, he's not going to pay $100 for a com- 
plete work-up. Even for a dead dog, I doubt 
whether he'll pay $100 to have a necropsy and 
bacteriology work-up on a dog. At least not at 
the University of Washington. They complained 
about the purchase price of a dog being over 
$40 or $50 in the first place. So, my guess is 
that we could not support this elaborate an ef- 
