Edema Formation in Guinea Pigs. 47 
animals which had received 26 and 80 injections, respectively, 
survived for seven and twenty-three months, and at autopsy 
showed no edema. Two which had received 47 and 70 injections, 
respectively, survived for twelve and nineteen months, and at 
autopsy showed marked ascites, over 30 c.c. of fluid being collected 
in the latter case. The remaining animal which received 86 in- 
jections survived for twenty-three months, and at autopsy showed 
marked ascites, and double hydrothorax. In all but one case in 
this series, death occurred in from two to five days after the last 
injection. 
In Group II, four animals which died within four months after 
the first injection, showed no edema. One which received 10 
injections of 5 m.grms. each, during fifteen months, died two days 
after the last injection, and showed marked subcutaneous edema, 
but no accumulation of fluid into the serous cavities. This animal 
increased 50 grms. in weight during the last two days of life. 
One animal which received eight injections during thirteen months, 
died five days after the last injection, and showed moderate sub- 
cutaneous edema, marked ascites, double hydrothorax, and hydro- 
pericardium. Still another which received four injections during 
eight months, died eleven days after the last injection, having 
increased 125 grms. in weight during the last five days of life, 
and at autopsy showed marked subcutaneous edema, and marked 
ascites, over 120 c.c. of ascitic fluid being collected. The remain- 
ing animal of this series received seven injections during fourteen 
months, died eight days after the last injection, and showed 
moderate ascites. 
In Group III, one animal which received two injections of 
10 m.grms. and one which received a single injection of 15 m.grms. 
died within four months, and showed no edema. One which re- 
ceived 30 m.grms. in three doses, died in sixteen months, eleven days 
after the last injection, and showed ascites, double hydrothorax, 
and hydropericardium. The remaining animal received 20 m.grms. 
in three injections, died in sixteen months, five days after the last 
injection and showed moderate subcutaneous edema, and moder- 
ate ascites. 
In the complete series of seventeen animals which survived 
for longer than three months, seven which died before the eighth 
