26 
Scientific Proceedings (40). 
The autopsy showed that the lungs, liver, kidneys, suprarenal 
capsule, spleen and glands were free from tubercular infection. 
Both controls lost weight consistently until death. 
The experimental rabbit held its weight up to a week before 
its death when it lost weight very rapidly. 
This rabbit appears to have been successfully protected by 
injections of tuberculin and tuberculous rabbit serum from a fatal 
dose of a culture of bovine tuberculosis. We are now duplicating 
this experiment with two experimental rabbits and three controls. 
On June 25, 19 10, two variations of the previous methods 
were used. Two rabbits were used for each experiment. Vari- 
ation 1 consisted in alternate injections of crude tuberculin and 
tuberculous rabbit serum as follows: 
June 25, each rabbit 
c. tuberculin. 
" 27, " 
< . 
2 
' tuberculous rabbit serum. 
" 28, " 
u 
H 
' tuberculin. 
" 29, " 
.-* 
2 
" tuberculous rabbit serum. 
" 30, " 
ii 
U 
" tuberculin. 
July 1, " 
<< 
2 
" tuberculous rabbit serum. 
" 2, " 
( I 
H 
1 tuberculin. 
.< . << 
4» 
(< 
2 
" tuberculous rabbit serum. 
«c _, «i 
5» 
( < 
M 
" tuberculin. 
" 6, " 
i < 
2 
" tuberculous rabbit serum. 
... 7> .. 
<< 
H 
" tuberculin. 
" 8, " 
<< 
2 
" tuberculous rabbit serum. 
Variation 2 consisted in alternate injections of tuberculous 
rabbit serum and that portion of the tubercle bacillus split by 
alcoholic potash which is insoluble in the alcohol. Vaughan 
claims that this portion of the tubercle bacillus sensitises but does 
not poison. This is true of the preparation we used as will be 
described further on. 
The following table gives the injections: 
June 25, each rabbit i/io gm. insoluble residue. 
27, 
2 c.c. tuberculous rabbit 
serum. 
" 28, " 
" 1/10 gm. insoluble residue. 
" 29, " 
2 c.c. tuberculous rabbit 
serum. 
" 30, " 
1/10 gm. insoluble residue. 
July 1, " 
2 c.c. tuberculous rabbit 
serum. 
" 2, " 
1/10 gm. insoluble residue. 
" 4, " 
2 c.c. tuberculous rabbit 
serum. 
" 5, " 
1/10 gm. insoluble residue. 
" 6, " 
2 c.c. tuberculous rabbit 
serum. 
1/10 gm. insoluble residue. 
" 8, " 
2 c.c. tuberculous rabbit 
serum. 
