94 
Scientific Proceedings (43). 
creatic juice from which the coagulable proteins were removed 
by heat caused an equally marked fall in the blood pressure. 
Extracts of the fresh pancreas were also prepared with the 
Buchner press or by simple grinding in a mortar with sand or glass 
and shaking with 0.85 per cent, salt solution. As a general rule 
3 c.c. of the resulting solutions representing I gm. of the organ 
were used for single injections. The results were similar whether 
the solution was used at once or was kept on ice for 18 to 24 hours. 
Before use the solutions were cleared as far as possible by centrifu- 
galization and filtering. Such extracts produced a fall in blood 
pressure averaging 30 to 50 mm. Hg. Similar results were obtained 
by using extracts from which the coagulable proteid was removed 
by heat and acetic acid, and also with the alcoholic precipitate of 
such a solution taken up in the original volume of 0.85 per cent, 
salt solution. 
The alcoholic filtrates of the preceding extracts evaporated to 
dryness at 37.5 0 C. and made up to the original volume are inert. 
Washed or unwashed organs give extracts of similar depressor 
activity. The clear solutions obtained after heating and acidi- 
fication were used to determine the effects of the injection of small 
amounts of extract over long periods of time. Death resulted 
generally from the injection of the equivalent of 3.5 to 6 grams of 
pancreas over a period of about one half hour, but in one instance 
an animal received the equivalent of 31 grams of pancreas, during 
two and one half hours, before death occurred. 
It was found impossible by continuous injection of small 
quantities to keep the blood pressure at the low level produced by 
a single injection of the equivalent of 1 gram of pancreas. The 
blood pressure remains but slightly below its original level, but if 
a large dose is then given there is a marked fall, with recovery 
to the level existing at the time of injection. Attempts to deter- 
mine the effects of continuous absorption of pancreatic juice from 
the peritoneal cavity by causing leakage from the severed ducts 
were also unsuccessful. 
That there might be some ground for drawing analogies be- 
tween experimental findings and clinical conditions, extracts 
were prepared from human pancreas and it was found that their 
action on the dog was in every way similar to that obtained with 
