Dextrose in the Exudate of Pulmonary Edema. 99 
injection. In 27 frogs the injected dose of fuchsin was less than o. 1 
mgr. per grm. of body-weight. In eighteen frogs the dose was 
0.05 mgm. per gram body-weight, the time of onset of convulsions 
varying between 4 and 15 minutes. In some of these cases the 
entire dose for the frog amounted to less than one milligram of 
the fuchsin. In a few frogs the effective dose was not more than 
0.025 mgm. per gram body-weight. 
We have here another instance in which the action of a sub- 
stance is greatly accelerated and much more effective in animals 
without a circulation than in normal animals. The experiments 
seem to show further that the minimum toxic dose of fuchsin is 
for cardiectomized frogs much smaller than even for frogs with the 
anterior part of the brain removed. 
60 (585) 
On the presence of dextrose in the exudate of pulmonary edema. 
By I. S. KLEINER and S. J. MELTZER. 
[From the Department of Physiology and Pharmacology of the 
Rockefeller Institute for Medical Research.] 
Injection of adrenalin causes glycosuria and hyperglycemia. 
In some instances the animals which receive adrenalin die of pul- 
monary edema. The chemical composition of the exudate of 
pulmonary edema has never been investigated. An examination 
of the exudate of rabbits which died from pulmonary edema 
after receiving adrenalin revealed the presence of a considerable 
amount of dextrose. The causation of pulmonary edema by the 
injection of adrenalin is, however, a matter of mere accident and 
cannot be relied upon in a systematic study. After various at- 
tempts we found that inhalation of ammonia can be fairly well 
relied upon to produce edema and produce it in a quantity suffi- 
cient to make a quantitative test for a reducing substance. The 
exudate did not clot, which shows that no pure blood was mixed 
with it. The number of experiments, although not yet large, per- 
mits a definite preliminary report. Besides analyzing the pulmo- 
nary exudate, in most cases a quantitative analysis of the blood for 
reducing substances was made and in some instances also of the 
urine. Pulmonary edema was also produced in two normal ani- 
