Inclusion of Carmine Particles by Sarcoma Cells, i i 3 
In order to compare the two methods more closely a second 
series of experiments were performed in which the individual 
remained clothed and the hand, or arm, in the plethysmograph 
was covered with water of the same temperature as the water 
that had been in the calorimeter. The results of these experiments, 
shown in Table II., agree with the first set. 
Table II. 
Average Room 
Temp. 
Average Calor- 
imeter. 
Rate by Calor- 
imeter. 
Plethysmograph Rate. 
Hand. 
Arm. 
v.z. 
v.z. 
v.z. 
24-5° 
24° 
25.5° 
29.8 0 
28.0° 
29.6 0 
7.4 
7.4 
4-7 
9.6 
8-3 
8.6 
3.1 
These observations indicate that the blood flow in the hand, 
relative to its volume, is faster than is the flow in the combined 
forearm and hand. We found considerable differences in the 
results obtained by the two methods used on the hand. We do 
not know the exact causes of these differences but are inclined 
to believe that both hand methods are subject to greater error 
than is the plethysmograph method when applied to the arm. 
69 (594) 
On the phagocytic inclusion of carmine particles by sarcoma 
cells growing in vitro with consequent staining of 
the cell granules. 
By P. M. HANES and R. A. LAMBERT. 
[From the Department of Pathology of the College of Physicians 
and Surgeons, Columbia University, New York.] 
The cells of rat and mouse sarcomata when cultivated in vitro 
show active amoeboid movements and wander for considerable 
distances into the surrounding plasma. Upon adding finely 
powdered carmine particles to the plasma medium the wandering 
tumor cells take up the particles in an active phagocytic manner. 
The carmine particles within the cells are easily distinguishable 
by their opacity and angularity. The carmine is partially dis- 
solved within the cell and brings into evidence the granules of the 
