TABLE III 
Biological Characters of some Organisms the Names of which were not definitely determined 
3 4 5 6 
Morphology 
Large cocci of different 
Majority occur in pairs, 
the points of contact being 
flattened. 
The diameter of the cocci 
i* about 1-5 M. 
Some arc found singly, 
others in tetrad*. 
In old cultures the cocci 
stain badly, and many large 
swollen-cut badics arc seen. 
Cocci spherical, some arc 
Thcyoccur singly, in pairs, 
and short chains. 
When in pairs the sides of 
contact arc flattened. 
They are about twice as 
large as staphylococcus au- 
Cultures resemble micro- 
Slender bacilli, l p to } yt 
Early formation of oval 
many cases to the slender 
This 'bacillus ha, a strong 
to the bacillus alvci. 
Straight rods of varying 
length, some growing out 
Early formation of spores; 
in the centre of the bacillus 
whilst at each pole is a small 
deeply-stained spot of pro- 
toplasm. 
Length of bacillus, 4 ft to 
Small spherical cdcci in 
masses, the siic of staphylo- 
Small slender bacillus, 
proteus. 
translucent, brown' colony! 
Margins radially striated. 
Raised, circular, orange- 
yellow, viscid growth. 
Translucent, flat, slightly- 
raited, whitish film, spread- 
ing quickly and tending to 
cover the surface of the 
Colonies brownish and 
Colonies are circular, opa- 
colour, they quickly extend 
in diameter, some becoming 
4 inch broad. 
Translucent, dirty-white 
colony, from the periphery 
of which processes arc given 
off. 
Pale, white, well-defined 
^ Centre whiter and more 
upaque than the margins. 
Surface becomes finely 
granular. 
Single colonies are rounded, 
circular, and translucent. 
with a coarsely granular sur- 
face. 
Piatt euliure,.— No deep 
On the surface a slightly- 
raised, translucent, spreading 
film. 
Streak.— As on serum the 
whole surface of the agar. 
Single colonies transpar- 
When confluent there is 
a translucent, white, slight- 
ly-raised growth spreading 
of short, rounded off-shoots, 
the edges of which are trans- 
parent and feathery. 
Streak. — In one day an 
abundant, raised, white 
growth, quickly acquiring 
The grow th extends later- 
ally. 
the edges arc well defined 
Deep colonies, elliptical 
On the surface grey, trans- 
houn; C X7e' increase In" 
siic and have a rounded cen- 
tre and margins made up of 
No grow th was obtained. 
Growth opaque, yellow, 
raised in the centre and not 
Surface o/growth dry. 
Along the line of inocu- 
lation a narrow, slightly- 
coloured growth with no 
tendency to spread laterally. 
Scanty growth, as a nar- 
row, slichtly-raiscd, yellow- 
ish line which with age 
Mttraitopital appearance. — 
The bacilli are much thicker 
Growl abundantly in the 
condensation water. 
In a short time the growth 
becomes slightly yellow. 
Light-brownish, smooth, 
translucent growth, which 
quickly acquires a reddish- 
Growth occurs in the con- 
Microscopically there are 
many thread forms. 
White sandy deposit. 
Slight general turbidity. 
No appreciable grow th. 
No appreciable growth. 
Finely granular turbidity 
and thick while deposit. 
General turbidity, form- 
ing in a week a thick, white 
scum and an abundant white 
deposit. 
Indol reaction. 
Glucose Bhotm 
Diiicminated growth and 
The organism grows an- 
aerobkally. 
General turbidity with an 
abundant white deposit and 
General turbiditv. 
No acid or gas formation. 
Litmus Milk 
No change in the medium. 
Yellowish deposited grow th 
No change ia the medium. 
There it a considerable 
amount of deposited growth, 
hut no coagulation of the 
Acid reaction. 
No coagulation of casein. 
After j to 4 days the lit 
S C038Ul3led 38 3 ,h,ck 
Fair amount of deposited 
Plait.— Colonies are min- 
ute.circular, and translucent, 
Ry re fleeted tight they have 
a light-brown, finely granu- 
lar appearance. 
Stab. — No growth in the 
depth. 
On the surface is a circu- 
which spreads slowly and 
Waw.-Cdonies minute, 
circular, opaque and orange 
yellow. 
Medium not liquefied. 
Streak. — Raised orange- 
yellow- growth. 
Slab. — Raised orange- 
vcllow growth on the sur- 
face ; in the depth a num- 
Streak. — Growth very 
slow .^appearing as 3 limited, 
ing laterally. 
Slab. — So growth in the 
depth. 
On the surface a whitish, 
Plait.— Colonies on the 
colonies), circular, and opa- 
11 Deep colonies are spheri- 
cal and white. 
white, irregular growth, the 
outer margins of w hich are 
Plan.— Minute, opaque- 
white, regular colonies, 
which by transmitted light 
ami a dark-brown nucleus. 
No liquefaction. 
The colonies in a short 
time become yellow. 
white growth with abrupt, 
Plate.— Minute^ opaque- 
SVrwi.— Railed, grey, sil- 
very-white growth, spread- 
ing a little laterally. 
Margins raised and well- 
Sireak. — White semi- 
develops a brownish-yellow 
The surface is dry and 
(lazed. 
Streak.— Growth occurs 
when planted thickly as a 
slowly . extending growth 
with a light-brownish cen- 
The surface is dry and 
slightly wrinkled or striated. 
The edges are crenate. 
No liquefaction. 
ber of opaque, light-yellow 
Slab. — In the depth a 
number of minute, opaque, 
w hile colonics. 
On the surface is a while 
growth, extending laterally 
in a number of short, fern- 
like off-shoots. 
well-defined margins. 
In a week the growth has 
become a brilliant fleah- 
Stab.— Opaque, cream or 
flesh-coloured expansion on 
the surface. 
In the depth thick grinu- 
time large whitish colonies 
with a brown nucleus dc- 
log a little laterally with a 
Margins finely serrate. 
Later the centre of the 
growth assumes a wrinkled 
Glucoit gtlaiint ,hakt— 
Urine 
Motility 
Stain isc 
Reactions 
No ammoniacal decom- 
Non-motile. 
Early cultures stain well 
with the aniline dyes ; old 
cultures take the stain badly. 
Stain by Gram's method. 
Stains well with all the 
Stains by Gram's method. 
Does not stain by Gram's 
method. 
Non-motile. 
Stains with nil the aniline 
dyes. 
Does not retain the stain 
by Cram's method. 
Non-motilc. 
Stains with aniline dyes, 
and by Gram's method. 
Motile. 
Stnins with aniline dyes. 
Does not stain by Gram's 
