1 I 2 
THOMPSON YATES LABORATORIES REPORT 
serum ; a little of this subculture was taken on the loop of a platinum wire and 
smeared over the whole surface of the rabbit's conjunctiva with gentle friction. 
Where this rule is departed from mention will be made. 
The experiments show that staphylococci from the normal conjunctival sac 
were considerably less virulent than similar staphylococci occurring in an inflamed 
conjunctiva. One loopful of a one day old agar culture of any of the staphylococci 
obtained from a healthy sac produced no reaction in a rabbit's conjunctiva when 
introduced without injury to the surface of the membrane, whereas simple introduction 
of staphylococcus aureus, and in one instance of staphylococcus albus, obtained from 
inflamed eyes was followed by a very appreciable inflammatory reaction. 
With gentle friction, however, a very severe reaction followed inoculation of 
pyogenic staphylococci obtained from inflamed eyes, whilst of the staphylococci 
obtained from the healthy conjunctiva, staphylococcus aureus was the only one which 
produced any marked reaction, and this reaction was decidedly less than the 
inflammation produced by a staphylococcus aureus derived from an inflamed eye. 
The following tables show at a glance the methods employed, and the results 
obtained, in each experiment. 
