II 
In the first part of this paper we have shown how it is possible to group the 
chief micro-organisms with regard to their behaviour and growth in Taurocholate- 
glucose broth. 
In order to further separate and distinguish the various members or each 
group, we have studied their fermentative power upon the following sugars, viz., 
glucose, lactose, mannite, and cane, upon glycerine, their reaction in litmus milk, 
and their growth on blood serum and Taurocholate-lactose agar. The results are 
shown below in tabular form. 
Temperature and length of time of incubation 
In this table in every instance the temperature of incubation has been 42 C. 
The duration of incubation has been forty-eight hours, except where otherwise stated. 
Nature of media employed 
The composition of the various media employed is as follows : — 
Litmus taurocholate glucose broth: Sod. taurocholate, o - 5 per cent.; 
glucose, o - 5 per cent. ; peptone, 2 - o per cent. ; water, 100 cc. 
Litmus glucose broth: Glucose, 0*5 per cent.; peptone, 2'0 per cent.; 
water, 100 cc. 
Litmus lactose broth: Lactose, 1 -o per cent.; peptone, 2"0 per cent.; 
water, 100 cc. 
Litmus mannite broth: Mannite, 1 -o per cent.; peptone, 2 - o per cent.; 
water, 100 cc. 
Litmus cane sugar broth: Cane sugar, i - o per cent. ; peptone, 2 - o per cent ; 
water, 1 00 cc. 
Litmus glycerine broth : Glycerine, i - o percent.; peptone, 2 - o per cent; 
water, 100 cc. 
Taurocholate lactose agar: Agar, 1.5 per cent.; sod. taurocholate, 0*5 per 
cent.; lactose, 1 - o per cent.; peptone, 2 - o per cent.; water, 
100 cc. 
