2o 4 THOMPSON YATES LABORATORIES REPORT 
Horse 3. Convalescent. The blood also clotted rapidly, but did not form 
so large a buffy coat as in the previous case. It was sterile. 
Horse 4. Suffering badly. The conjunctivae were greatly injected and a 
serous fluid was discharged from'both eyes. The blood was tested and found to be 
sterile. A cover slip preparation was made from the eye discharge, it showed small 
bacilli and a tetra-coccus. Agar culture tubes and Petri dishes were inoculated with 
the eye discharge, and from this we obtained an almost pure culture of what, to save 
repetition, we called the characteristic bacillus, because of the uniformity with which 
it was met with in this and the following cases. It was a small diplococcus or diplo- 
bacillus which grew as a white opaque streak on gelatine, causing no liquefaction. 
On agar it grew more vigorously and slightly more opaque than B. coli. In glucose 
gelatine it formed gas. It showed motility in a hanging-drop preparation. The 
culture of one of the agar tubes was suspended in sterile water, and three guinea-pigs 
were inoculated with equal portions of the suspension. Two days afterwards one of 
the guinea-pigs had an oedematous swelling at the point of inoculation, it was killed, 
and there was found considerable subcutaneous oedema containing small diplococci. 
Agar tubes were inoculated from the oedematous fluid, and from the heart blood ; 
those from the latter showed the characteristic bacillus both culturally and micro- 
scopically. 
Horse 5. Suffering badly. Cultures from the conjunctiva showed the 
characteristic bacillus. A guinea-pig inoculated with some of the culture died in 
three days with an oedematous swelling similar to the last, and a cultivation from 
this gave the same bacillus. 
Horse 6. Cultures were made from the lungs, trachea, nasal mucous mem- 
brane, conjunctivae, liver, spleen, kidney. The characteristic bacillus was recovered 
from the whole of the respiratory tract, but not from the organs. A guinea-pig 
inoculated with a culture from the trachea died in three days ; the subcutaneous 
oedematous fluid showed the same characteristic bacillus, and cultivations were 
obtained from this and also from the blood. Guinea-pigs were also inoculated with 
cultures from the nasal mucous membrane, and from the conjunctivae with similar 
results. Other guinea-pigs were inoculated with the subcutaneous oedematous fluid 
and these died under similar conditions. Three guinea-pigs which had four days 
previously received an inoculation of 5 c.c. of blood serum from horse 1 were in- 
oculated with a suspension of an agar plate culture of the nasal mucous membrane ; 
three fresh guinea-pigs were also inoculated with a similar amount as controls. One 
of the controls died in two days under similar conditions as the above. 
Horse 7. Suffering badly, cultures were made from the conjunctival discharge, 
and showed the characteristic bacillus. Afterwards, when the horse died, cultures 
were made from the nasal mucous membrane, bronchus of healthy lung, bronchus 
of pneumonic lung, and from the pneumonic lung substance. The same bacillus 
