A NEW PATHOGENIC BACIELUS 
399 
2. The statement of Lehmann and Neumann that B. enteritidis clots milk 
is based entirely upon LubarschV 5 paper ; no other observer has 
noted a similar result. On the contrary, it is an accepted charac- 
teristic of the entire B. enteritidis group, that they do not clot 
milk. 
^. As shown by Durham, 9 the members ot the B. enteritidis group are 
not agglutinated by the serum of animals immunized with B. coli. 
The present study has led to the conclusion that because the Neutral-red reaction 
occurs, bacilli capable of causing typhoid-like symptoms cannot be excluded, for every 
member of the B. enteritidis group has been found to give the characteristic reaction. 
In order to test the matter thoroughly, two series of media were prepared ; one con- 
taining glucose, the other lactose, in view of the fact that the latter sugar is fermented 
by B. coli, and not by the other groups. To each medium was added one per cent, 
of a saturated watery solution of Neutral-red. Three strains of B. typhosus, nine of 
the B. enteritidis group, and three of B. coli, as well as a culture of B. faecalis alkali- 
genes, were used for comparison. The results are shown in the following table :— 
TABLE VI 
Shewing the comparative action of five groups in Sugar media containing I per cent, saturated 
aqueous solution of Neutral-red. 
o'5 Per Cent. Glucose Media 
48 Hours : 37 C. 
o - 5 Per Cent. Lactose Media 
4S Hours : 37 C. 
Agar 
Shake 
Bouillon 
Peptone 
Water 
Agar 
Shake 
Peptone 
Water 
B. typhosus 
O 
T A 
Slight T A 
O 
T Alk. 
B. enteritidis 
GDF 
T A 
T A 
D F 
T Alk. 
Bacillus ' L ' 
GDF 
T A 
T A 
D F 
T Alk. 
B. coli 
G D F 
T A 
T A 
GDF 
T A 
B. faecalis alkaligenes ... 
0 
T Alk 
0 
0 
T Alk. 
o, no change. G, gas. D, decolourization. F, fluorescence. T, turbidity. A, acid. Alk, alkaline. 
