442 THOMPSON YATES LABORATORIES REPORT 
I am indebted to Dr. Barr for the temperature chart and clinical history. 
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The condition of the intestinal tract suggested enteric fever. Plate cultures 
were therefore made on agar and MacConkey's bile salt agar from the spleen, a 
mesenteric gland and a swollen Peyer's patch. The spleen contained B. typhosus 
in pure culture, the mesenteric gland a mixture of B. typhosus and B. coli ; from the 
the Peyer's patch the B. typhosus was not isolated, probably because the bacteriological 
examination was made twenty-eight hours after death. 
The following media were employed in the identification of the typhoid bacillus: — 
glucose, lactose, mannite, and cane sugar broth, bouillon, litmus milk, gelatine and 
potato. 
The results of the cultivation were characteristic in the nine cultures worked out 
from the plate colonies, with the exception that three gave a trace of indol in bouillon 
after forty-eight hours incubation. The bacillus was decolourized by Gram's method. 
It was agglutinated by the sera of typhoid patients in a dilution of one in thirty. 
Dr. Hume found that it was also agglutinated by the serum of a guinea pig 
immunized against typhoid in a dilution of one in two hundred. Cultures were 
also highly pathogenic when inoculated into the peritoneal cavity of guinea pigs. 
The use of bile salt agar plates assisted me greatly in the differentiation of B. 
typhosus from B. coli. 
Microscopical examination of the swollen Peyer's patches and solitary follicles 
demonstrated some increase in the number of lymphocytes, also an extensive proli- 
feration and desquamation of the endothelial cells derived from the reticulum of the 
