TRYPANOSOME OCCURRING IN THE BLOOD OF MAN 459 
3. Oedema, more especially about the eyes. 
4. Injection of the skin and sometimes conjunctivae. 
5. Enlargement and tenderness of the spleen. 
6. Constant frequent pulse and respirations (hurried breathing). These 
symptoms associated with no definite organic lesion. 
The Parasites observed in the Blood 
Although many slides were made and fresh preparations of the blood examined 
throughout the time the patient was under observation, no malaria parasites were 
discovered. 
In fresh blood the parasite is a very minute worm-like body, very difficult to see 
with a magnification of three hundred diameters ; especially is this the case when 
only few are present in a preparation, and the parasite is amongst a clump of red 
corpuscles ; it glides along fairly rapidly in among the red cells, imparting very little 
movement to them. When the movements have slowed down it is seen that one 
end is drawn out into a whip-like process — the flagellum ; the other end is bluntly 
conical ; attached along the body is a flange-like process — the undulating mem- 
brane ; the body itself is short and thick, and its substance granular. There is a highly 
refractile spot situated near the posterior end (Vacuole). 
Movements : The parasite usually is seen progressing with the flagellum (anterior 
end) in front, but at times when an obstruction is insurmountable, it shoots backwards 
for a short distance with the blunted end (posterior) in front. Slow progression is 
brought about by wave-like motions started in the flagellum and communicated 
along the undulating membrane. The parasite in rapid motion moves in a screw-like 
manner, its body rotating around the longitudinal axis so that the undulating mem- 
brane appears as if it were spirally arranged around the organism. This appearance is 
seen in specimens of blood preserved in two per cent, formol in normal saline. 
When movements slow down, I have observed on two or three occasions, 
parasites, apparently attached by their posterior end to a red corpuscle indenting its 
capsule by the waves sent along the undulating membrane. I have never observed 
the red corpuscle damaged in this wav. On one occasion I observed the process of 
phagocytosis take place on a slide one hour after the blood was drawn ; a mono- 
nuclear leucocyte had partially englobed the trypanosome, only the flagellum and a 
small portion of the anterior part of the body remaining free. 
In fresh preparations, ringed with vaseline, the parasites appear to die in a few 
hours after the blood is drawn (one observation three hours). In such preparations, 
left over night, I was never able to find the trypanosome again in the morning. 
Atmospheric temperature varied from 90 0 in the day to 65 0 during the night. I 
was unable to obtain an exact measurement of the parasite in the fresh state. 
