SYNTHESIS OF FATS ACCOMPANYING INTESTINAL ABSORPTION 31 
Weight of fatty acid = 0*0042 gramme. 
,, neutral fat (as olein)= 0*1031 ,, 
Percentage of neutral fat =96*1 
,, fatty acid = 3'9 
Experiment 4. A dog, weighing 12*4 kilogrammes, was fed with 100 grammes 
of olive oil at 9 a.m., and the fatty chyle was collected at 3 p.m. (interval six hours), by 
means of a pipette drawn out to a capillary end, from the mesenteric surface after 
puncture of the lacteals, into a small porcelain dish. The lymph was weighed and 
r.nalysed as before, with the following results : - 
Weight of lymph = 1 "871 2 gramme. 
,, ethereal extract = 0*1450 ,, 
„ fatty acid = 0*0042 ,, 
,, neutral fat (as olein) = 0*1326 ,, 
Percentage of neutral fat = 96*9 
„ fatty acid = 3*1 
Accordingly, the fatty matter present in the mesenteric lacteal vessels is proven 
to consist practically all (upwards of 95 per cent.) of neutral fat, demonstrating that 
the synthesis from the products of digestion occurs in the intestinal wall. 
ExPhRIMENTS ON THE NATURE OF THE FaTTY CONSTITUENTS IN THE INTESTINAL 
Mucosa during Fat Absorption 
Immediately after the collection of the lymph used in the preceding series of 
experiments, the animals were killed, and the entire small intestine was removed, cut 
open longitudinally, and thoroughly washed with water to remove adherent matter. 
The intestine was then laid out with the mucous surface upward on a glass plate, and 
the mucosa rubbed off with the back of a knife. The scraped off mucous membrane 
was weighed and extracted first with a mixture of alcohol and ether (1 alcohol to 3 of 
ether), and then with ether alone. The solvents were decanted off, the solutions 
mixed, and evaporated to dryness. The dry residue was next extracted with dry 
ether, filtered, and the ethereal extract evaporated to dryness. The residue was 
finally weighed, and the amount of fatty acid and fat estimated. 
Experiment 1. Total weight of moist mucous membrane = 31*7 grammes ; 
weight of total ethereal extract = 1*1524 gramme ; weight of fatty acid by titration 
= 0*1802 gramme; weight of neutral fat by difference = 0*9722 gramme ; relative 
percentages of neutral fat and fatty acid = 84*3 and 15*7. 
Experiment 2. Weight of moist mucosa = 14*4 grammes ; weight of total 
ethereal extract = 0*8074 gramme ; weight of fatty acid, by titration = 0*2904 
gramme ; weight of neutral fat, by saponification and titration = 0*5303 gramme; 
relative percentage of neutral fat and fatty acid = 64*6 and 35*4. 
