34 THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
emulsion, and some drops of material insoluble in the fluid were found floating on 
the surface, while under the low power of the microscope a large number of oily 
globules of varying size were visible. 
Experiment 2. The abdominal lymphatic glands of the same cat, which was 
used for experiment i, were taken, and likewise digested at 33 0 C. for ninety hours in 
presence of chloroform, with four times their weight of normal saline. The extract 
was filtered and centrifugalized, giving a clear reddish-yellow fluid in which no 
cellular elements were visible under the microscope. 
Ten cubic centimetres of this fluid were taken, o - 2 gramme of sodium oleate, 
and 0*052 gramme of glycerine were added, and the clear fluid obtained on warming 
to 38-5° C. and agitating, was digested in a bath at 38-5° C. 
The experiment was started at 6 p.m., and next morning, at 10.30 a.m., 
(interval = i6h. 30m.) there was a thick layer of vellow-coloured oil on the surface 
which formed a temporary emulsion on shaking, and the fluid gave under the low 
power a field filled with globules, closely resembling milk as seen under the 
microscope. 
The strength of the lymphatic gland extract in this particular experiment 
judging from the depth of the oily layer, was much greater than that of the extract 
of intestinal mucosa, but later experiments showed that no general law to this effect 
applied. 
The oily layer here formed looked so like olive oil, and the appearance of 
the globules under the microscope so closely resembled that of fat globules as seen 
in milk, that it was at first thought that olein had been synthesized from the sodium 
oleate and glycerine added to the extract ; but a determination of the amount of free 
fatty acid in the oil soon dispelled this illusion. 
The contents of the test tube were extracted by shaking four times with an 
equal volume of ether. In this process, it was observed not only that the oily layer 
disappeared on the first addition of ether, but also in the shaking up with this first 
portion of ether, the aqueous layer became quite clear. The four portions of ether, 
after separation from the aqueous layer, were united, the ether was evaporated off, and 
the residue weighed (wt. = 0-1188 gramme). The residue was then dissolved in 
warm alcohol, and the solution was titrated with deci-normal sodic hydrate, using 
rosolic acid as an indicator, 3*7 c.c. were required for neutralization, indicating 
0'i040 gramme of oleic acid. Hence 87-5 per cent, of the soap decomposed by the 
extract had gone to form oleic acid and not neutral fat. 
It might be thought that the development of oleic acid here occurring was 
due to acidity of the lymphatic extract employed, but a portion of the same lymphatic 
extract, which had, therefore, also been ninety hours in the incubator, was tested 
with rosolic acid and found to be alkaline, the alkalinity being equivalent to of 
sodic hydrate. 
