36 THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
After this procedure, the eight tubes were placed in a water bath at 3 6° C, 
and examined at intervals. 
Intestine. Examined one-and-a-half hours later, all four of the intestinal tubes 
showed turbidity, and, under the microscope, thickly studded fields of oily globules. 
Examined after the lapse of nineteen hours thirty-five minutes, all four of 
the intestinal extracts show a thick creamy layer on top, a little less marked on the 
boiled tube, No. 4, than on the others, but still very obvious. Under the micro- 
scope all four show fields crowded with oil globules. The reaction of the contents of 
all lour tubes is still distinctly alkaline to phenol-phthalein. 
Pancreas. The four tubes containing pancreatic extract examined one hour 
after the commencement of the digestion show in each case a milky fluid, and the 
microscope demonstrates abundance of oily globules. All four tubes give an alkaline 
reaction to phenol-phthalein, but less strongly marked than in the case of the 
intestine. The action obviously is more intense here than with the intestinal extracts. 
Examined at the end of eighteen hours, there is a thick layer of oil and cream at the 
top of each tube, which is not apparently any less in the boiled tube than in the other 
three tubes. 
Microscopic examination shows in all four tubes oily globules of all sizes, and in 
great number. There are apparently no fewer globules in No. 4 than in any of the 
other tubes. 
The four pancreatic tubes are all still alkaline to phenol-phthalein. 
The eight tubes were then extracted with ether, and the weight of ethereal 
extractive, and amount of free fatty acid determined as above described ; the results 
are given in the following table : — 
Intestinal Mucosa 
Contents ot tubes 
Weight of 
ethereal extract 
Weight of free 
oleic acid 
Percentage of 
free oleic acid 
No. I. Oleate 4 glycerine + dextrose ... 
No. 2. Oleate + glycerine 
No. 3. Oleate alone 
No. 4. Oleate alone, then boiled... 
cr I 800 1 
cr 1258 
0-1582 
0-1322 
O" I 664 
0-1256 
0-1466 
o- 1 269 
92.4 
99-8 
92-7 
96-0 
No. 
1. 
Oleate 4 glycerine 4 dextrose .. 
0-2688 
0-2580 
96-7 
reas 
No. 
2. 
Oleate 4 glycerine 
0-2568 
0 - 23I2 
90-0 
Pane 
No. 
3- 
Oleate alone 
0-2 I 76 
0-2 I40 
98-3 
No. 
4- 
Oleate alone, then boiled... 
0-2394 
0-2284 
95'+ 
1. The weighings were taken to J of a milligramme. 
